PMID: 4388856Jan 1, 1969

Synthesis and induction of tyrosine aminotransferase in synchronized hepatoma cells in culture

Proceedings of the National Academy of Sciences of the United States of America
D W MartinDaryl K Granner

Abstract

The generation cycle of an established line of rat hepatoma cells (HTC cells) was studied using synchronized cell techniques. Dexamethasone phosphate (Dex), a synthetic adrenal corticosteriod which induces tyrosine aminotransferase (TAT) in HTC cells randomly distributed in the cell generation cycle, did not affect the durations of the various phases of the cycle. The activities of TAT and several dehydrogenases, and the rates of general protein and RNA synthesis, were studied throughout the cycle.Dex, at 10(-5)M, when added to a synchronized cell population in the latter two thirds of G1 phase or anywhere in the S phase, induces TAT. During the period made up of G2, M, and early G1, Dex does not induce TAT. However, radioisotope incorporation into specifically immunoprecipitated TAT continues during mitosis, when general protein and RNA synthesis are decreased.

References

Jan 1, 1966·Cold Spring Harbor Symposia on Quantitative Biology·G M TomkinsB Peterkofsky
Jul 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·E B ThompsonJ F Curran
Jul 28, 1968·Journal of Molecular Biology·Daryl K GrannerG M Tomkins
Apr 1, 1963·Experimental Cell Research·T TERASIMA, L J TOLMACH

Citations

Feb 1, 1970·Biochemical Genetics·J B Wyngaarden
Jan 1, 1970·In Vitro·R Sinclair
Jan 1, 1984·The International Journal of Biochemistry·J V GroenewaldW Oelofsen
Mar 1, 1979·Journal of Steroid Biochemistry·M Sluyser, A Tulp
Nov 1, 1983·Journal of Steroid Biochemistry·P MarchettiS Iacobelli
Aug 19, 1971·Biochimica Et Biophysica Acta·J HanouneA Josipowicz
Dec 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·J E BodwellA Munck
Aug 12, 1998·The Journal of Steroid Biochemistry and Molecular Biology·J E BodwellA Munck
Mar 18, 1976·The New England Journal of Medicine·T D Gelehrter
Jul 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·D W MartinM A Bresler
Apr 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·D W Martin, G M Tomkins
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J M VargaA B Lerner
Dec 1, 1972·Plant Physiology·A OaksD Stevens
Feb 1, 1971·Biological Reviews of the Cambridge Philosophical Society·R T Johnson, P N Rao
Sep 28, 1973·Science·J E DarnellG R Molloy
Aug 11, 2011·PloS One·Laura MatthewsDavid Ray
Jun 18, 1971·Biochemical and Biophysical Research Communications·M SmulsonC Rideau
Oct 1, 1981·Experimental Cell Research·G Wegener, H W Sauer
Jun 1, 1970·Experimental Cell Research·H J Burki, C A Tobias
Sep 28, 1972·Journal of Molecular Biology·H WeintraubH Holtzer
May 5, 1975·Biochemical and Biophysical Research Communications·P P McCannF Schuber
Jan 1, 1986·Alcoholism, Clinical and Experimental Research·R T Cook, G S Martin
Dec 4, 1969·Biochemical and Biophysical Research Communications·F C BancroftA H Tashjian
Apr 21, 1975·Biochemical and Biophysical Research Communications·J R Emanuel, T D Gelehrter
Aug 1, 1969·FEBS Letters·J J.M. BergeronC A. Pasternak
Feb 1, 1979·Journal of Cellular Physiology·J M HarmonE B Thompson
Dec 1, 1981·Journal of Cellular Physiology·O Bakke, K B Eik-Nes
Aug 1, 1973·Journal of Cellular Physiology·P Liberti, C Bagioni
Jun 1, 1970·Journal of Cellular Physiology·S E PfeifferG Sato
Jun 1, 1988·Journal of Cellular Physiology·S B Horowitz, Y T Lau
Jul 15, 1975·European Journal of Biochemistry·J Woodward, M J Merrett
Mar 17, 1975·European Journal of Biochemistry·T Eremenko, P Volpe
Jun 1, 2002·Molecular Endocrinology·G Alexander AbelTheo Rein
Apr 1, 1981·Die Naturwissenschaften·D Gallwitz
Jun 11, 2002·The Journal of Biological Chemistry·Elisabeth D Martinez, Mark Danielsen
Jul 26, 2015·The Journal of Biological Chemistry·Daryl K Granner

Related Concepts

Adrenal Cortex Hormones
Oradexon
Enzyme Induction
Liver Carcinoma
Malignant Neoplasm of Liver
Neoplasm Proteins
RNA, Neoplasm
Tyrosine Aminotransferase
Culture Techniques

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