Synthesis and optimization of wide pore superficially porous particles by a one-step coating process for separation of proteins and monoclonal antibodies

Journal of Chromatography. a
Wu ChenXiaoli Wang

Abstract

Superficially porous particles (SPPs) with pore size ranging from 90Å to 120Å have been a great success for the fast separation of small molecules over totally porous particles in recent years. However, for the separation of large biomolecules such as proteins, particles with large pore size (e.g. ≥ 300Å) are needed to allow unrestricted diffusion inside the pores. One early example is the commercial wide pore (300Å) SPPs in 5μm size introduced in 2001. More recently, wide pore SPPs (200Å and 400Å) in smaller particle sizes (3.5-3.6μm) have been developed to meet the need of increasing interest in doing faster analysis of larger therapeutic molecules by biopharmaceutical companies. Those SSPs in the market are mostly synthesized by the laborious layer-by-layer (LBL) method. A one step coating approach would be highly advantageous, offering potential benefits on process time, easier quality control, materials cost, and process simplicity for facile scale-up. A unique one-step coating process for the synthesis of SPPs called the "coacervation method" was developed by Chen and Wei as an improved and optimized process, and has been successfully applied to synthesis of a commercial product, Poroshell 120 particles, for small molecul...Continue Reading

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Citations

Apr 3, 2019·Annual Review of Analytical Chemistry·Yu LiangYukui Zhang
Dec 4, 2019·Molecular & Cellular Proteomics : MCP·Daniela SalasLeonard J Foster
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Daniela SalasLeonard J Foster
Dec 11, 2020·Analytical Chemistry·K Broeckhoven, G Desmet
Nov 9, 2018·Analytical Chemistry·Valentina D'AtriDavy Guillarme

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