PMID: 6160263Oct 1, 1980Paper

Synthesis and processing of polymerase proteins of wild-type and mutant avian retroviruses

Journal of Virology
R N EisenmanM Linial

Abstract

We have studied the biosynthesis of avian retrovirus proteins related to reverse transcriptase in permissive avian embryonic cells. Analysis of immune precipitates from avian sarcoma virus (ASV)-infected cells demonstrated the presence of the 180,000-dalton gag-pol "read-through" protein (Pr180gag-pol) and a 130,000-dalton polypeptide (Pr130gag-pol). Pr130gag-pol was found, in serological and peptide mapping studies, to consist primarily of sequences related to reverse transcriptase and the gag-encoded protein p15. Pr180gag-pol was found to be phosphorylated, whereas Pr130gag-pol was not. In addition, only Pr180gag-pol but not Pr130gag-pol was susceptible to cleavage with the virion protease p15. Although the structure of Pr130gag-pol would suggest that it is generated by removal of a portion of the gag region from Pr180gag-pol, an analysis of labeling kinetics has failed to demonstrate unequivocally whether Pr130gag-pol is a cleavage product of Pr180gag-pol or a primary translation product. We were repeatedly unable to detect either Pr180gag-pol or Pr130gag-pol in virus particles released from the cell, whereas both beta and alpha subunits were readily observed. Several presumed intermediates between Pr130gag-pol and the beta ...Continue Reading

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Citations

Jan 1, 1992·Journal of Enzyme Inhibition·A L DeVico, M G Sarngadharan
Feb 1, 1992·AIDS Research and Human Retroviruses·P Li, C J Burrell
Mar 14, 2021·Communications Biology·Krishan K PandeyDuane P Grandgenett
Jun 1, 1986·Journal of Virology·D P GrandgenettJ C Olsen
Aug 1, 1992·Journal of Virology·J LubanS P Goff
Oct 1, 1981·Journal of Virology·S M Anderson, J H Chen
Apr 1, 1992·Journal of Virology·S R MummD P Grandgenett
Oct 1, 1990·Journal of Virology·L StewartV M Vogt
Nov 1, 1984·Molecular and Cellular Biology·S R Hann, R N Eisenman
Aug 1, 1988·Journal of Virology·M KotlerA M Skalka
Dec 1, 1989·Journal of Virology·C ChenM L Linial
Mar 1, 1982·Virology·W S MasonJ M Taylor

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