PMID: 7033933Dec 11, 1981Paper

Synthesis and processing of ribosomal RNA in isolated yeast mitochondria

Nucleic Acids Research
P BoernerT D Fox

Abstract

The synthesis and processing of the 15S and 21S rRNAs have been studied in isolated yeast mitochondria. When mitochondrial transcripts were labeled with [alpha-32p]UTP in an incubation mixture containing 50 microM ATP, the transcripts from the genes for the large and small ribosomal RNAs accumulated in the form of putative precursor molecules. The labeled pre-21S rRNA was converted to mature 21S rRNA during a chase period in the presence of 1 mM ATP. Thus, the maturation of 21S rRNA, a process which includes trimming at the 3' end and, in omega+ strains, the excision of a 1.1 kb intervening sequence, can occur in isolated mitochondria and appears to be dependent on ATP. In contrast, the maturation of 15S rRNA by the removal of approximately 80 nucleotides from the 5' end of a 15.5S transcript is severely restricted in isolated mitochondria, even in the presence of 2.5 mM ATP.

References

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Citations

Apr 26, 2000·International Journal for Parasitology·K T MilitelloL K Read
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P M Finnegan, G G Brown
Apr 1, 1986·European Journal of Biochemistry·R Docherty, D Rickwood
May 4, 1987·European Journal of Biochemistry·T LisowskyG Michaelis
Jan 1, 1983·CRC Critical Reviews in Biochemistry·H F TabakP Borst
Jun 30, 1987·Biochemical and Biophysical Research Communications·G R KulkarniN G Avadhani
Jun 1, 1995·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·H DaiK S Chiang

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