PMID: 6988212Jan 1, 1980Paper

Synthesis and properties of N-acetyl-N'-(p-glyoxylylbenzoyl)cystamine, a new reagent for RNA-RNA and RNA-protein cross-linking

European Journal of Biochemistry
A Expert-Bezançon, D Hayes

Abstract

The synthesis of N-acetyl-N'-(p-glyoxylylbenzoyl)cystamine (Gbz-Cyn2-Ac) is described. Like other glyoxal-type reagents it reacts with guanine and arginine. The kinetics and pH dependence of these reactions are studied. (Gbz-Cyn2-Ac) reacts with non-base-paired guanines at about 20 sites in 16-S rRNA. After reduction of disulfide bonds each derivatized guanine residue carries a free -- SH group which can be used to create RNA-RNA bridges or, after introducing an additional photoactivatable derivative, RNA-protein bridges.

References

Sep 7, 1979·Journal of Theoretical Biology·H P Yockey
Mar 30, 1978·Biochemical and Biophysical Research Communications·R I Glazer, A L Peale
Jun 5, 1977·Journal of Molecular Biology·A Expert-BezançonD H Hayes
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Apr 6, 1978·Molecular & General Genetics : MGG·E UlmerR Brimacombe
Sep 1, 1978·European Journal of Biochemistry·H G BäumertC G Kurland
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Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P L WollenzienJ E Hearst
Mar 30, 1978·Biochemical and Biophysical Research Communications·J D Glass, M Pelzig
Nov 1, 1978·Nucleic Acids Research·R Wagner, R A Garrett
Nov 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H F Noller, J B Chaires
Jan 1, 1974·Biochimie·A Expert-BezançonJ Thibault
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Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P C JelencS R Simon

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Citations

Nov 2, 1983·European Journal of Biochemistry·A Expert-BezançonP Carbon
Jan 1, 1982·Progress in Biophysics and Molecular Biology·C Hélène, G Lancelot

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