PMID: 737185Dec 21, 1978Paper

Synthesis and properties of O6-methyldeoxyguanylic acid and its copolymers with deoxycytidylic acid

Biochimica Et Biophysica Acta
J R Mehta, D B Ludlum

Abstract

This paper describes the synthesis of O6-methyldeoxyguanosine triphosphate (m6dGTP) and its copolymerization to high molecular weight polymer with deoxycytidylic acid. The monomer, m6dGTP, was synthesized from deoxyguanosine first protected by acetylation of the sugar hydroxyls, and then chlorinated in the 6-position with POCl3. The product, 6-chloro-3',5'-di-O-acetyl deoxyguanosine, was converted to O6-methyldeoxyguanosine with sodium methoxide and phosphorylated in the 5' position with carrot phosphotransferase. Monophosphate was converted chemically to the triphosphate and copolymerized with dCTP by terminal deoxynucleotidyl transferase. The resulting template, which contained O6-methylguanine, was tested for its ability to direct RNA synthesis by bacterial RNA polymerase. The presence of O6-methylguanine was shown to lead to the misincorporation of UMP in the product polymer, thus strengthening the hypothesis that O6-methylguanine is a promutagenic base.

References

Oct 1, 1971·Biochimica Et Biophysica Acta·D B Ludlum
May 10, 1973·Biochimica Et Biophysica Acta·L L Gerchman, D B Ludlum
Sep 15, 1970·Biochemistry·B Singer, H Fraenkel-Conrat
Jul 31, 1972·Biochimica Et Biophysica Acta·L L GerchmanD B Ludlum
Aug 1, 1970·Analytical Biochemistry·C L HarveyA L Nussbaum

❮ Previous
Next ❯

Citations

Jan 1, 1984·Journal of Cancer Research and Clinical Oncology·H M RabesR Wilhelm
Jan 1, 1983·Journal of Cancer Research and Clinical Oncology·H M Rabes
Apr 1, 1980·Biochemical Pharmacology·W G Verly
Jan 1, 1984·Mutation Research·P F Schendel, I Michaeli
Jan 1, 1985·Advances in Enzyme Regulation·H M RabesT Bücher
Jul 8, 2009·Organic & Biomolecular Chemistry·Mahesh K Lakshman, Josh Frank
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J R MehtaW G Verly
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B SingerJ T Kuśmierek
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B SingerH Fraenkel-Conrat
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C L GreenJ M Essigmann
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E L LoechlerJ M Essigmann
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L D Williams, B R Shaw
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K S EllisonJ M Essigmann
Dec 1, 1987·Environmental Health Perspectives·B W GlickmanP A Burns
Jun 28, 2007·The Journal of Organic Chemistry·Soon Bang KangMahesh K Lakshman
Mar 1, 1983·Mutation Research·R S FooteS Mitra
Jan 1, 1990·Environmental and Molecular Mutagenesis·M J HorsfallB W Glickman
Jan 1, 1981·Progress in Biophysics and Molecular Biology·S A Kumar
Mar 28, 1996·Chemical Reviews·Marek Gniazdowski, Cinzia Cera
Apr 1, 1990·Archives of Biochemistry and Biophysics·C AuclairI Emerit
Oct 9, 1989·Journal of Theoretical Biology·R H Duncan, G S Davies
Feb 1, 2003·The Journal of Organic Chemistry·Zlatko JanebaMorris J Robins

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.