Synthesis and proteomic activity evaluation of a new isotope-coded affinity tagging (ICAT) reagent

Bioconjugate Chemistry
Annalisa GuaragnaGiovanni Palumbo

Abstract

During recent years, quantitative proteome profiling has taken advantage of incorporating the traditional stable isotope dilution analysis into global scale or discovery-based proteomic experiments that use mass spectrometers as detectors to allow the pairwise study of differently expressed proteins. Quantitative protein analysis by means of the isotope-coded affinity tag (ICAT) method and tandem mass spectrometry (MS) enables the pairwise comparison of protein expression levels in biological samples. Herein, a modified ICAT reagent, named BAA-ICAT (beta-alanine-arm-ICAT) in which the polyether linker is replaced by a more water-soluble polyamide one, was investigated.

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Citations

Nov 30, 2011·Bioconjugate Chemistry·Annalisa GuaragnaGiovanni Palumbo
Oct 18, 2011·Expert Review of Proteomics·Kevin M Coombs
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria De FenzaAnnalisa Guaragna
Jun 24, 2021·Bioconjugate Chemistry·Adam P WdowiakMatthew J Piggott

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