Synthesis and release of Hageman factor (Factor XII) by the isolated perfused rat liver

The Journal of Clinical Investigation
H SaitoA Angell

Abstract

The site of synthesis of Hageman factor (HF, Factor XII) has not been previously demonstrated with certainty. We have studied the production and release of HF in the isolated perfused rat liver and have compared rates of synthesis in this system with absolute rates of degradation measured in vivo. Rat livers, perfused for 5 h with a recycling fluid consisting of a perfluorochemical emulsion (Fluosol 43), were used to demonstrate a cumulative increase of HF in the perfusate as measured by a specific and sensitive radioimmunoassay. The rate of increase in the perfusate pool of HF during the final 4 h of perfusion yielded a mean synthetic rate of 3.5 micrograms/h per 100 g body wt, which was approximately 0.2% of the synthetic rate of albumin in the same system. The cumulative appearance of albumin and transferrin was linear after 1 h and calculated rates of synthesis were 2,012 micrograms/h per 100 g and 263 micrograms/h per 100 g body wt, respectively. De novo synthesis of HF was confirmed by demonstrating incorporation of [14C]lysine into specific immunoprecipitates of HF, and by the observations that both specific incorporation of labeled amino acid and net release of immunoassayable HF were inhibited by the administration of ...Continue Reading

References

Jul 1, 1978·The Journal of Clinical Investigation·G H GoldsmithO S Ratnoff
May 17, 1977·Biochemistry·K FujikawaE W Davie
Jul 1, 1977·British Journal of Haematology·A G Morton, A S Tavill
Nov 11, 1975·Thrombosis Research·C A Owen, E J Bowie
Jan 1, 1966·International Archives of Allergy and Applied Immunology·P J McConahey, F J Dixon
Dec 1, 1974·The Journal of Clinical Investigation·C R McMillinA G Walton
Oct 1, 1974·The Australian Journal of Experimental Biology and Medical Science·M E Gardiner, E H Morgan
Sep 1, 1965·Immunochemistry·G ManciniJ F Heremans
Nov 1, 1969·Archives of Biochemistry and Biophysics·M Steinbuch, R Audran
Apr 27, 1971·Biochimica Et Biophysica Acta·P Righetti, J W Drysdale
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H SaitoO D Ratnoff

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Citations

Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·E M GordonF L Hall
Jan 19, 2012·Journal of Immunological Methods·Peter C PapageorgiouJohn S Floras
Jun 15, 1989·Thrombosis Research·G M PatrassiA Girolami
Jan 6, 2012·Clinical Science·Peter C PapageorgiouJohn S Floras
Oct 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W L HuE Regoeczi

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