PMID: 9547267Jun 20, 1998Paper

Synthesis and RNA polymerase incorporation of the degenerate ribonucleotide analogue rPTP

Nucleic Acids Research
K MoriyamaDavid Loakes

Abstract

The synthesis and enzymatic incorporation into RNA of the hydrogen bond degenerate nucleoside analogue 6-(beta-d-ribofuranosyl)-3, 4-dihydro-8H-pyrimido[4,5-c]-[1,2]oxazin-7-one (P) is described. The 5'-triphosphate of this analogue is readily incorporated by T3, T7 and SP6 RNA polymerases into RNA transcripts, being best incorporated in place of UTP, but also in place of CTP. When all the uridine residues in an HIV-1 TAR RNA transcript are replaced by P the transcript has similar characteristics to the wild-type TAR RNA, as demonstrated by similar melting temperatures and CD spectra. The P-substituted TAR transcript binds to the Tat peptide ADP-1 with only 4-fold lowered efficiency compared with wild-type TAR.

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Citations

Jan 14, 2010·Analytical Chemistry·Gudrun StengelRobert D Kuchta
Nov 30, 2006·Nucleic Acids Research·Tetsuya SuzukiKazuo Negishi
Aug 10, 2007·Journal of Virology·Jason D GraciCraig E Cameron
Dec 23, 2011·Journal of Virology·Jason D GraciCraig E Cameron
Apr 13, 2004·Antiviral Chemistry & Chemotherapy·Jason D Graci, Craig E Cameron
Oct 21, 2020·International Journal of Molecular Sciences·Katsuhito KinoHiroshi Miyazawa

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