Synthesis and secretion of insulin-like growth factor (IGF)-II and IGF binding protein-2 by cultivated brain meningeal cells

Brain Research
K IshikawaK Tatemoto

Abstract

The meninges (the pia mater and the arachnoid) covering the surface of brain parenchyma are known to participate in the regulation of blood circulation and the blood-cerebrospinal fluid barrier. In the present study, we isolated and characterized some of the major proteins secreted into the conditioned medium of the meningeal cell cultures prepared from 1-2-day-old rats. Five protein molecules (7 kDa, 16 kDa, 18 kDa, 27 kDa and 32 kDa) were recognized as major proteins in the conditioned medium by SDS-PAGE. The major proteins were isolated and purified to homogeneity, respectively. Their N-terminal sequences, except that of 27 kDa protein, were successfully determined. Homology search has revealed that the N-terminal sequences of the 7 kDa protein and the 32 kDa protein were identical with those of insulin-like growth factor-II (IGF-II) and IGF-binding protein-2 (IGFBP-2), respectively. The N-terminal sequences of the 16 kDa and 18 kDa molecules were identical with those of the corresponding fragments of IGFBP-2. The present study demonstrates that cultured meningeal cells produce and secrete large amounts of IGF-II and IGFBP-2. The meninges may, therefore, be one of the main sources of these proteins in the cerebrospinal fluid...Continue Reading

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Citations

Sep 1, 2005·Endocrine Reviews·V C RussoG A Werther
Feb 24, 2001·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·S OginoM L Cohen
Apr 17, 2004·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Takeshi MurakiKoichi Ishikawa
Feb 11, 2011·Tissue Engineering. Part a·Cornelius H LamAllison Hubel
Jan 27, 2015·Journal of Cell Communication and Signaling·Steven W YauVincenzo C Russo

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