PMID: 8589741Jan 1, 1996Paper

Synthesis and secretion of the mouse whey acidic protein in transgenic sheep

Transgenic Research
R J WallL Hennighausen

Abstract

The synthesis of foreign proteins can be targeted to the mammary gland of transgenic animals, thus permitting commercial purification of otherwise unavailable proteins from milk. Genetic regulatory elements from the mouse whey acidic protein (WAP) gene have been used successfully to direct expression of transgenes to the mammary gland of mice, goats and pigs. To extend the practical usefulness of WAP promoter-driven fusion genes and further characterize WAP expression in heterologous species, we introduced a 6.8 kb DNA fragment containing the genomic form of the mouse WAP gene into sheep zygotes. Two lines of transgenic sheep were produced. The transgene was expressed in mammary tissue of both lines and intact WAP was secreted into milk at concentrations estimated to range from 100 to 500 mg/litre. Ectopic WAP gene expression was found in salivary gland, spleen, liver, lung, heart muscle, kidney and bone marrow of one founder ewe. WAP RNA was not detected in skeletal muscle and intestine. These data suggest that unlike pigs, sheep may possess nuclear factors in a variety of tissues that interact with WAP regulatory sequences. Though the data presented are based on only two lines, these findings suggest WAP regulatory sequences ...Continue Reading

References

May 1, 1992·Transgenic Research·A ShamayL Hennighausen
Jan 1, 1992·Cancer Treatment and Research·R A McKnightL Hennighausen
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·W H VelanderW N Drohan
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R J WallL Hennighausen
Nov 1, 1991·Journal of Animal Science·A ShamayR J Wall
Dec 1, 1991·The Quarterly Review of Biology·D M Hillis, M T Dixon
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A C AndresP Gerlinger
Nov 26, 1984·Nucleic Acids Research·S M CampbellA E Sippel
Apr 24, 1982·Nucleic Acids Research·L G Hennighausen, A E Sippel
Jun 15, 1982·European Journal of Biochemistry·L G Hennighausen, A E Sippel

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Citations

Mar 23, 2000·Theriogenology·J D Murray
Jun 6, 2000·Advanced Drug Delivery Reviews·W A Marasco, Dana Jones S
Nov 13, 2004·The Journal of Nutritional Biochemistry·P A PrietoB Kelder
Oct 1, 1996·Current Opinion in Biotechnology·Y Echelard
Dec 22, 1999·Genetic Analysis : Biomolecular Engineering·F O CastroO Hayes
Oct 6, 1997·Journal of Dairy Science·R J WallK R Bondioli

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