PMID: 9445398Mar 7, 1998Paper

Synthesis and secretion of the pancreatic-type carboxyl ester lipase by human endothelial cells

The Biochemical Journal
F Li, D Y Hui

Abstract

Human aortic extracts contain significant cholesteryl ester hydrolytic activity. The enzymic activity was shown to be activated by trihydroxylated bile salt, but not by dihydroxylated bile salt. Monospecific antibodies prepared against rat pancreatic carboxyl ester lipase (CEL, cholesterol esterase) immunoprecipitated cholesteryl ester hydrolytic activity from human aorta, demonstrating that the neutral CEL in aorta is highly similar to and probably identical with the pancreatic enzyme. Reverse transcriptase PCR amplification of mRNA from human aortic endothelial cells revealed de novo synthesis of the pancreatic-type CEL by these cells. Preincubating human aortic endothelial cells with oxidized or native low-density lipoprotein resulted in an 8- and 3-fold increase in CEL activity secreted into the culture medium respectively. A potential physiological role for the endothelial CEL was demonstrated by studies showing its ability to confer partial protection against the cytotoxic effects of lysophosphatidylcholine. The protective effect of CEL is related to its bile-salt-independent lysophospholipase activity. However, CEL hydrolysis of lysophosphatidylcholine can be inhibited by excess cholesterol. Taken together, these results...Continue Reading

Citations

Jun 8, 2007·Experimental Eye Research·Chuan-Ming LiChristine A Curcio
Aug 17, 1999·Annual Review of Nutrition·I Tabas
Apr 21, 2005·Pancreas·Kyoko MiyasakaAkihiro Funakoshi
Jun 23, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Stefano La RosaCarlo Capella
Apr 13, 2000·Journal of Internal Medicine·M O PentikäinenP T Kovanen
Dec 1, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Ouafa RebaïAlain Vérine
Jun 25, 2003·Circulation·Nathalie AugéAlain Vérine
Sep 9, 2006·Current Opinion in Lipidology·Katariina Oörni, Petri T Kovanen
Nov 27, 2007·The Journal of Clinical Investigation·Laurence Panicot-DuboisChristophe Dubois
Dec 11, 2008·Virchows Archiv : an International Journal of Pathology·Stefano La RosaCarlo Capella
Jul 12, 2002·The Journal of Biological Chemistry·Emeline AubertDominique Lombardo
Jul 26, 2017·Interdisciplinary Sciences, Computational Life Sciences·George Kadakasseril VargheseSolomon Habtemariam
Jul 13, 1999·Free Radical Biology & Medicine·V P PalaceP K Singal
Aug 22, 2001·Biochimica Et Biophysica Acta·D Lombardo

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