Synthesis and solution-phase conformation of the RG-I fragment of the plant polysaccharide pectin reveals a modification-modulated assembly mechanism.

Journal of the American Chemical Society
Eoin M ScanlanBenjamin G Davis

Abstract

The syntheses of pure RG-I fragments of key plant matrix biomolecule pectin using a counterintuitive late-stage convergent cis-glycosylation has allowed detailed analyses of their solution-phase conformations, metal binding affinities, pK(a) values, self-assembly equilibria, and diffusional kinetics. These reveal a striking, right-handed 3(1)-helix that provides an effective and repeating lateral display of putative liganding carboxylates. Moreover, these heteropolymeric structures allow units as short as tetrasaccharides to self-assemble through carbohydrate-carbohydrate interactions that are induced by the presence of Ca(II), a known dynamic trigger in planta. These self-assembly properties can be switched simply by the addition or removal of a single methyl group in this repeating unit through methyl (de)esterification, another known dynamic trigger in planta. Together, the combined effect of Ca(II) and methylation revealed here suggests a concerted molecular basis for these two major dynamic modifications in planta.

References

May 5, 2000·Carbohydrate Research·M MaruyamaH Yamada
Aug 23, 2003·Annals of Botany·Philip J White, Martin R Broadley
Apr 2, 2008·Carbohydrate Research·Navid NematiChristian Vogel
Nov 11, 2008·Biochemistry. Biokhimii︠a︡·M A ProtsenkoN P Korableva
Jun 11, 2009·Plant Signaling & Behavior·Narendra Tuteja, Sudhir K Sopory
Jul 21, 2009·Carbohydrate Research·Kerry Hosmer Caffall, Debra Mohnen

❮ Previous
Next ❯

Citations

Dec 1, 2017·Plant Cell Reports·Jason N BurrisC Neal Stewart
Feb 7, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alexandra N ZakharovaMads H Clausen
Jan 16, 2014·Organic & Biomolecular Chemistry·Jean BourkeEoin M Scanlan
Nov 5, 2016·Angewandte Chemie·Benjamin SchumannPeter H Seeberger
Aug 10, 2017·Chemical Reviews·Christine KinnaertMads H Clausen
Apr 3, 2013·Organic Letters·Alexandra N ZakharovaMads H Clausen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
John A CrumpFrederick J Angulo
American Journal of Epidemiology
N M MirzaW M Macharia
Lancet
Jennifer BryceWHO Child Health Epidemiology Reference Group
Nature Reviews. Microbiology
Michinaga OgawaChihiro Sasakawa
© 2021 Meta ULC. All rights reserved