Synthesis and structural organization of zonular fibers during development and aging

Matrix Biology : Journal of the International Society for Matrix Biology
E HanssenR Garrone

Abstract

Zonular fibers are a specific form of extracellular matrix composed mainly of fibrillins. The purpose of this study was to determine which cells secrete fibrillin-1 during development and aging. A specific guinea pig fibrillin-1 mRNA probe was designed and cloned in order to identify fibrillin-secreting cells in guinea pig eye, using in situ hybridization. Immunofluorescence, with a specific guinea pig monoclonal antibody, was used to compare protein levels at different stages from birth to 35 months of age. Electron microscopy and immunolabeling were used to investigate the organization of zonular microfibril bundles. We identified the cells of non-pigmented epithelium of the ciliary body as the main source of fibrillin secreted into the zonule. Moreover, while mRNA expression decreased during aging, there was no decrease in fibrillin immunoreactivity, as previously described in human aorta. These data indicate a very slow turnover of the zonular microfibrils which can be correlated with the appearance during aging of a new periodic fibrillar structure. This new structure may reflect an increased cross-linking in the long-lived zonular microfibrillar bundles.

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Citations

Mar 16, 2013·Investigative Ophthalmology & Visual Science·Yanrong ShiSteven Bassnett
Sep 9, 2008·Experimental Eye Research·Brian P Danysh, Melinda K Duncan
Mar 15, 2005·Veterinary Ophthalmology·Rebecca A Morris, Richard R Dubielzig
May 11, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Dirk Hubmacher, Suneel S Apte
Sep 9, 2006·Experimental Eye Research·H R L Napier, S H Kidson
Oct 28, 2008·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Brian P DanyshMelinda K Duncan
May 20, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Leonoor I LosJohanna M M Hooymans
Jun 22, 2018·Histochemistry and Cell Biology·Felke SteijnsMarjolijn Renard
Dec 20, 2007·Current Opinion in Ophthalmology·Pascal Dureau
Sep 5, 2006·American Journal of Veterinary Research·Audrone R BikneviciusHilary M Clayton
May 27, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·JodiRae DeDreuA Sue Menko
Jul 24, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Mari HiraokaMasahiko Takada
Oct 12, 2020·Matrix Biology : Journal of the International Society for Matrix Biology·Y ShiS Bassnett
Mar 2, 2021·Investigative Ophthalmology & Visual Science·Xiaowei YuZhigang Fan
Sep 28, 2020·Progress in Retinal and Eye Research·Steven Bassnett
Feb 10, 2009·American Journal of Human Genetics·Dina AhramHatem El-Shanti

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