PMID: 16637292Apr 28, 2006Paper

Synthesis and structure-function study of artificial nucleases on the basis of hemin conjugates with peptide fragments of cell differentiation factor HLDF

Bioorganicheskaia khimiia
G A ZheltukhinaI A Kostanian

Abstract

A series of octa-hexapeptide fragments of HLDF and their conjugates with hemin were obtained by solid phase peptide synthesis. A relationship between the structure and the nuclease activity of the compounds was established. The effect of various factors (medium pH, the presence of metal ions, complexons, reducers, and buffer composition) on DNA destruction with hemin peptides was studied. Preliminary information confirming an oxidative mechanism of this process was obtained. The cleavage of plasmid DNA under the action of hemin peptides was studied by the methods of electron microscopy, gel electrophoresis, and atomic force microscopy.

References

Oct 12, 1976·Calcified Tissue Research·R A Peckauskas, I Pullman
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·N C HortonJ J Perona
Aug 2, 2003·Biochemical and Biophysical Research Communications·R MukhopadhyayS Verma

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