PMID: 7083049Mar 1, 1982Paper

Synthesis and turnover of proteins and mRNA in germinating wheat embryos

Canadian Journal of Biochemistry
Z F GrzelczakB G Lane

Abstract

The most prominent methionine-labeled protein made when cell-free systems are programmed with bulk mRNA from dry wheat embryos has been identified with what may be the most abundant protein in dry wheat embryos. The protein has been brought to purity and has a distinctive amino acid composition, Gly and Glx accounting for almost 40% of the total amino acids. Designated E because of its conspicuous association with early inhibition of dry wheat embryos, the protein and its mRNA are abundant during the "early" phase (0--1 h) of postimbibition development, and easily detected during "lag" phase (1--5 h), but they are almost totally degraded soon after entry into the "growth" phase of development, by about 10 h postimbibition. The most prominent methionine-labeled protein peculiar to the cell-free translational capacity of bulk mRNA from "growth" phase embryos is not detected as a product of in vivo synthesis. Its electrophoretic properties and its time course of emergence, after 5 h postimbibition development, suggest that this major product of cell-free synthesis may be an in vitro counterpart to a prominent methionine-labeled protein made only in vivo, by "growth" phase embryos. Designated G because of its conspicuous associatio...Continue Reading

Citations

Dec 1, 1993·Plant Molecular Biology·T S FutersA C Cuming
May 1, 1992·Plant Molecular Biology·J C LittsR L Rodriguez
Oct 1, 2008·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Paramjit KhuranaAnju K Chhibbar
Jan 20, 2004·Trends in Plant Science·Michael J Wise, Alan Tunnacliffe
Apr 24, 1987·Nucleic Acids Research·J C LittsR S Quatrano
Sep 1, 1991·Plant Physiology·W J HurkmanC K Tanaka
May 15, 2012·Biochimica Et Biophysica Acta·Takao FurukiMinoru Sakurai
Jul 19, 2014·Biochimica Et Biophysica Acta·Takao Furuki, Minoru Sakurai
May 2, 2016·Biochimica Et Biophysica Acta·Takao Furuki, Minoru Sakurai
May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Cristel CarlesMichel Delseny
Jul 1, 1990·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·T S FutersA C Cuming
Nov 10, 2006·The Plant Journal : for Cell and Molecular Biology·Shaheen B MowlaFrederica L Theodoulou
Oct 15, 1985·European Journal of Biochemistry·J D WilliamsonA C Cuming
Dec 19, 2006·Plant Biotechnology Journal·Agnelo Furtado, Robert James Henry
Jan 1, 1997·DNA Sequence : the Journal of DNA Sequencing and Mapping·S Van Campenhout, G Volckaert
Dec 24, 2011·International Journal of Microbiology·Sarah Rebecca PorterClaude Saegerman
Feb 6, 2003·The Journal of Biological Chemistry·Kshamata GoyalAlan Tunnacliffe
Jan 1, 1991·DNA Sequence : the Journal of DNA Sequencing and Mapping·J C LittsR S Quatrano

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Cell-Free System
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