Synthesis and utilization of E. coli-encapsulated PEG-based microdroplet using a microfluidic chip for biological application

Biotechnology and Bioengineering
Kyoung G LeeSeok Jae Lee

Abstract

We report herein an effective strategy for encapsulating Escherichia coli in polyethylene glycol diacrylate (PEGDA) microdroplets using a microfluidic device and chemical polymerization. PEGDA was employed as a reactant due to the biocompatibility, high porosity, and hydrophilic property. The uniform size and shape of microdroplets are obtained in a single-step process using microfluidic device. The size of microdroplets can be controlled through the changing continuous flow rate. The combination of microdroplet generation and chemical polymerization techniques provide unique environment to produce non-toxic ways of fabricating microorganism-encapsulated hydrogel microbeads. Due to these unique properties of micro-sized hydrogel microbeads, the encapsulated E. coli can maintain viability inside of microbeads and green fluorescent protein (GFP) and red fluorescent protein (RFP) genes are efficiently expressed inside of microbeads after isopropyl-β-D-thiogalactopyranoside induction, suggesting that there is no low-molecular weight substrate transfer limitation inside of microbeads. Furthermore, non-toxic, gentle, and outstanding biocompatibility of microbeads, the encapsulated E. coli can be used in various applications including...Continue Reading

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Citations

Sep 17, 2011·Analytical and Bioanalytical Chemistry·Elisa Michelini, Aldo Roda
Jul 10, 2012·ACS Nano·Kyoung G LeeTae Jung Park
Nov 1, 2012·Sensors·Byeong Il KimChang-Soo Lee
Dec 20, 2014·Chemistry & Biology·Christian PitzlerUlrich Schwaneberg
Apr 30, 2013·Polymers·Seila SelimovićAli Khademhosseini
Jul 13, 2018·Chemical Society Reviews·Wen LiDavid A Weitz
Apr 3, 2012·Small·Diego VelascoEugenia Kumacheva
Apr 8, 2014·Lab on a Chip·Kyun Joo ParkSeok Jae Lee
Apr 9, 2020·Frontiers in Bioengineering and Biotechnology·Fengxuan HanBin Li
Feb 1, 2019·Advanced Biosystems·Shrikrishnan Sankaran, Aránzazu Del Campo
Nov 26, 2013·Lab on a Chip·Dietmar Puchberger-EnenglMichael J Vellekoop
Nov 6, 2020·Lab on a Chip·Yuxin ZhangShi-Yang Tang
Nov 9, 2019·Environmental Science & Technology·Aaron BrewerYongqin Jiao

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