PMID: 9540838Apr 16, 1998Paper

Synthesis, antimicrobial activity and gene structure of a novel member of the dermaseptin B family

Biochimica Et Biophysica Acta
Y FleuryP Nicolas

Abstract

Dermaseptins are a family of cationic (Lys-rich) antimicrobial peptides that are abundant in the skin secretions of the arboreal frogs Phyllomedusa bicolor and P. sauvagii. In vitro, these peptides are microbicidal against a wide variety of microorganisms including Gram-positive and Gram-negative bacteria, yeasts, protozoa and fungi. To date, 6 dermaseptin B mature peptides, 24-34 residues long, 2 dermaseptin B cDNAs and 2 gene sequences have been identified in P. bicolor. To assess dermaseptin related genes further, we screened a P. bicolor genomic library with 32P-labeled cDNAs coding either for prepro-dermaseptins B1 or B2 (adenoregulin). A gene sequence was identified that coded a novel dermaseptin B, termed Drg3, which exhibits 23-42% amino acids identities with other members of the family. Analysis of the cDNAs coding precursors for several opioid and antimicrobial peptides originating from the skin of various amphibian species revealed that the 25-residue preproregion of these preproforms are all encoded by conserved nucleotides encompassed by the first coding exon of the Drg3 gene. Synthetic dermaseptin Drg3 exhibited a bactericidal activity towards several species of mollicutes (wall-less eubacteria), firmicutes (Gram-...Continue Reading

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Citations

Apr 3, 2004·Folia Microbiologica·P SímaK Sigler
Jun 11, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Milan OsuskySantosh Misra
Sep 17, 1999·International Journal of Food Microbiology·S Brul, P Coote
Apr 6, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Luciano P SilvaCarlos Bloch
Oct 22, 2008·Biochimica Et Biophysica Acta·Pierre Nicolas, Chahrazade El Amri
Dec 3, 2014·Peptides·Enrico KönigChris Shaw
Oct 20, 2010·Peptides·Enrico König, Olaf R P Bininda-Emonds
Jul 6, 2000·Bioscience, Biotechnology, and Biochemistry·S MachidaY Yu
Jul 17, 2018·Current Medicinal Chemistry·Jiri PatockaKamil Kuca

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