PMID: 6162481Jan 20, 1981Paper

Synthesis by DNA polymerase I on bleomycin-treated deoxyribonucleic acid: a requirement for exonuclease III

Biochemistry
O Niwa, R E Moses

Abstract

phi X174 RFI DNA treated with bleomycin (BLM) under conditions permitting nicking does not serve as a template-primer for Escherichia coli DNA polymerase I. Purified exonuclease III from E. coli and extracts from wild-type E. coli strains are able to convert the BLM-treated DNA to suitable template-primer, but extracts from exonuclease III deficient strains are not. Brief digestion by exonuclease III is enough to create the template-primer, suggesting that the exonuclease III is converting the BLM-treated DNA by a modification of 3' termini. The exonucleolytic rather than the phosphatase activity of exonuclease III appears to be involved in the conversion. Comparative studies with micrococcal nuclease indicate that BLM-created nicks do not have a simple 3'-P structure. Bacterial alkaline phosphatase does not convert BLM-treated DNA to template-primer. The endonuclease VI activity associated with exonuclease III does not incise DNA treated with BLM under conditions not allowing nicking, in contrast to DNA with apurinic sites made by acid treatment, arguing that conversion does not require the endonuclease VI action on uncleaved sites.

References

Feb 1, 1976·Antimicrobial Agents and Chemotherapy·S L Ross, R E Moses
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Oct 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·R E Moses, C C Richardson

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Citations

Sep 1, 1989·Mutation Research·L F Povirk, R J Steighner
Jan 1, 1989·Pharmacology & Therapeutics·M L VeiglW D Sedwick
Jan 30, 1990·Biochimica Et Biophysica Acta·M E Hagensee, R E Moses
Jun 1, 1983·Mutation Research·M M HurtR E Moses
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S SharmaR E Moses
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L F Povirk, I H Goldberg
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K SidikG A Freyer
May 25, 1992·Nucleic Acids Research·T A WintersT J Jorgensen
Dec 1, 2001·American Journal of Respiratory Cell and Molecular Biology·Y H HeW J Martin
Sep 1, 1990·Mutation Research·P W Doetsch, R P Cunningham
Dec 1, 1983·Journal of Biomolecular Structure & Dynamics·J K Barton
Jan 1, 1983·Drug Metabolism Reviews·K E Mc LaneK Ramakrishnan
Jan 1, 1988·Environmental and Molecular Mutagenesis·L F Povirk, C W Houlgrave
Dec 1, 2011·EcoSal Plus·Susan T Lovett

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