Synthesis, Characterization, and Applications of Nanographene-Armored Enzymes

Methods in Enzymology
Maryam KhanShamoon Asmat

Abstract

The unexpected discovery of graphene and especially the follow-up explosion of interest in its properties and applications marked the beginning of a new carbon era. Graphene-based nanostructured materials are highly useful because they show great promise in the field of biotechnology and biomedicine. Owing to their unique structural features, exceptional chemical, electrical, and mechanical properties, and their ability to affect the microenvironment of biomolecules, graphene-armored nanomaterials are suitable for use in various applications, such as immobilization of enzymes, field-effect transistors, photovoltaic devices, and biosensors, which in turn is extremely vital to the development of biomedical instruments, clinical diagnosis, and disease treatment. In this chapter, we present our recently reported work to armor hydrolytic enzymes on graphene-based nanomaterial in order to develop novel scaffolds to build robust nanobiocatalysts. Synthesized graphene-Fe3O4 and polyaniline-coated silver graphene nanocomposite have been used to immobilize β-galactosidase and lipase, using noncovalent and covalent strategies, respectively. Herein, the methodologies of both techniques have been discussed in detail. Owing to the large surf...Continue Reading

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