Synthesis, characterization, and in vitro cell culture viability of degradable poly(N-isopropylacrylamide-co-5,6-benzo-2-methylene-1,3-dioxepane)-based polymers and crosslinked gels

Journal of Biomedical Materials Research. Part a
Daniel J SiegwartKrzysztof Matyjaszewski

Abstract

Poly(N-isopropylacrylamide-co-5,6-benzo-2-methylene-1,3-dioxepane) (poly(NIPAAm-co-BMDO)) was synthesized by atom transfer radical polymerization (ATRP) and reversible addition-fragmentation chain transfer (RAFT) polymerization. Using UV-vis spectroscopy, the lower critical solution temperature (LCST) of poly(NIPAAm) and poly(NIPAAm-co-BMDO) copolymers were measured, varying with respect to the amount of incorporated BMDO. This material is degradable and possesses a LCST above room temperature and below body temperature, making it a potential candidate for use as an injectable tissue engineering scaffold to enhance fracture repair. ATRP and RAFT enabled preparation of polymers with control over molecular weight up to M(n) = 50,000 g/mol and M(w)/M(n) < 1.2. Degradation studies were performed in basic solution and in complete Dulbecco's modified Eagle medium. The cytotoxicity of the material and its degradation products were analyzed by in vitro cell culture analyses, including cytotoxicity live/dead and CyQUANT cell proliferation assays. Crosslinked scaffolds with degradable units within the polymer backbone and at the crosslinking sites were prepared using an ester-containing diacrylate crosslinker. Furthermore, incorporation ...Continue Reading

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Citations

Jan 1, 2012·Progress in Polymer Science·Daniel J SiegwartKrzysztof Matyjaszewski
Nov 29, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Leda Klouda
Sep 11, 2012·Journal of Biomedical Materials Research. Part a·Anna GalperinBuddy D Ratner
Dec 18, 2013·Macromolecular Rapid Communications·Vianney DelplaceJulien Nicolas
Sep 24, 2015·Nature Chemistry·Vianney Delplace, Julien Nicolas
May 6, 2015·European Polymer Journal·Caitlin G Decker, Heather D Maynard
Oct 16, 2016·Biomacromolecules·Samantha J PaluckHeather D Maynard
Feb 7, 2017·Chemical Society Reviews·Nane VanparijsBruno G De Geest
Dec 19, 2017·Macromolecular Rapid Communications·Uland Y LauHeather D Maynard
Jan 24, 2014·Journal of Periodontal & Implant Science·Sidi A BencherifPhilippe Renaud
Sep 24, 2010·Nature Chemistry·Mona Semsarilar, Sébastien Perrier
Dec 20, 2020·International Journal of Molecular Sciences·Si-Ting LinChih-Feng Huang
Jan 17, 2015·ACS Applied Materials & Interfaces·Joyce C BregerDavid H Gracias
Sep 13, 2014·Journal of the American Chemical Society·Emma M Pelegri-O'DayHeather D Maynard

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