Synthesis, conjugation, and immunological evaluation of the serogroup 6 pneumococcal oligosaccharides

Chembiochem : a European Journal of Chemical Biology
Archana R ParameswarAlexei V Demchenko

Abstract

The first synthesis of the newly discovered oligosaccharide of pneumococcal serotype 6C and its spacer-containing analogue is reported. Conjugation of the spacer-containing oligosaccharides of pneumococcal saccharides 6A, 6B, 6C and derivatives thereof with bovine serum albumin (BSA) protein carrier was carried out by using squaric-acid approach to obtain the oligosaccharide-protein conjugates in excellent yields. The conjugates have been tested with a rabbit antiserum pool (Pool B) used for pneumococcal serotyping. The results showed that synthetic carbohydrate conjugates express epitopes found in native capsular polysaccharides of serotypes 6A, 6B, and 6C.

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Citations

Jan 21, 2014·Chemistry & Biology·Chakkumkal AnishPeter H Seeberger
Feb 10, 2017·Chembiochem : a European Journal of Chemical Biology·Peng XuPavol Kovác
Apr 1, 2012·Chemistry of Heterocyclic Compounds·Scott J Hasty, Alexei V Demchenko
Jul 7, 2018·Beilstein Journal of Organic Chemistry·Aritra ChaudhuryRina Ghosh

Related Concepts

Metazoa
Antibodies, Bacterial
Antigenic Specificity
Bos indicus
Enzyme-Linked Immunosorbent Assay
Glycoconjugates
Mice, Inbred C57BL
Oligosaccharides
Polysaccharides, Bacterial
Chinchilla Rabbits

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