Abstract
Herein, we report the synthesis and characterization of a bishomoleptic and a trisheteroleptic ruthenium (II) polypyridyl complex, namely, [Ru(bpy)2(2, 2'-pq)](PF6)2 (1) and [Ru(bpy) (phen) (2, 2'-pq)](PF6)2 (2), respectively, where bpy = 2,2'-bipyridine, phen = 1,10-phenanthroline, and 2, 2'-pq = 2-(2'-pyridyl)-quinoxaline. The complexes were characterized by elemental analysis, TGA, 1H-NMR, FT-IR, UV-Vis, emission spectroscopy, and electrochemistry. Their structures were confirmed by single-crystal X-ray diffraction analysis. Complexes 1 and 2 were crystalized in orthorhombic, Pbca, and monoclinic, P21/n systems, respectively. Various spectroscopic techniques were employed to investigate the interaction of both complexes with calf thymus DNA (CT-DNA). The experimental data were confirmed by molecular docking studies, employing two different DNA sequences. Both complexes, 1 and 2, bind with DNA via a minor groove mode of binding. MTT experiments revealed that both complexes induce apoptosis of MCF-7 (breast cancer) cells in low concentrations. Confocal microscopy indicated that 2 localizes in the nucleus and internalizes more efficiently in MCF-7 than in HEK-293.
References
Oct 6, 1992·Biochemistry·S SatyanarayanaJ B Chaires
Oct 9, 1973·Biochemistry·J R Lakowicz, G Weber
Feb 24, 2001·Inorganic Chemistry·A Ambroise, B G Maiya
Jul 31, 2003·Nucleic Acids Research·Swapan S JainNicholas V Hud
Oct 7, 2004·Biophysical Journal·Nichola C GarbettDavid E Graves
Dec 17, 2005·Dalton Transactions : an International Journal of Inorganic Chemistry·Declan MulhernJohannes G Vos
Jan 30, 2007·Inorganic Chemistry·Dik-Lung MaKwok-Yin Wong
Sep 11, 2007·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Chunyu QiaoWeihong Zhou
Dec 15, 2007·Nature Protocols·Nichola C GarbettJonathan B Chaires
Dec 25, 2007·Acta Crystallographica. Section A, Foundations of Crystallography·George M Sheldrick
Apr 1, 2008·Inorganic Chemistry·Seann P MulcahyEric Meggers
Dec 22, 2009·Dalton Transactions : an International Journal of Inorganic Chemistry·Carlos Sánchez-Cano, Michael J Hannon
Jul 17, 2010·Bioinorganic Chemistry and Applications·Christiana A Mitsopoulou, Constantinos Dagas
May 10, 2011·Journal of Molecular Structure·Floyd A BeckfordNavindra P Seeram
Jun 24, 2011·Inorganic Chemistry·An-Guo ZhangChun-Hui Huang
Nov 30, 2013·International Journal of Pharmaceutics·Eleni K EfthimiadouGeorge Kordas
May 23, 2014·Inorganic Chemistry·Dennis L AshfordThomas J Meyer
Jul 12, 2014·European Journal of Medicinal Chemistry·Joana A PereiraMónica Vieira
Nov 14, 2014·Journal of the American Chemical Society·Bryan A AlbaniClaudia Turro
Jan 22, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Guoliang LiaoHui Chao
Jul 19, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Guanying LiHui Chao
Nov 4, 2016·Chemical Society Reviews·Yong-Jun YuanZhi-Gang Zou
Jan 4, 2017·Inorganica Chimica Acta·Jessica K WhiteClaudia Turro
Jan 12, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Konstantinos YpsilantisAchilleas Garoufis
Jan 14, 2017·Chemical Society Reviews·Noémie ElgrishiMarc Fontecave
Dec 6, 2017·European Journal of Medicinal Chemistry·Sana TariqMohd Amir
Oct 3, 2018·Journal of the American Chemical Society·Karan AroraJeremy J Kodanko
Dec 7, 2018·Bioorganic & Medicinal Chemistry·Tanu KaushalArvind Singh Negi
Sep 27, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Monize M da SilvaAlzir A Batista
Oct 1, 2019·Nucleosides, Nucleotides & Nucleic Acids·Hong-Ju YinKe-Zhi Wang
Mar 29, 2020·Journal of Biomolecular Structure & Dynamics·Selvan NehruSankaralingam Arunachalam
Jul 23, 2020·European Journal of Medicinal Chemistry·Jincan ChenTao Wang
Sep 4, 2020·Bioinorganic Chemistry and Applications·Jincan ChenLanmei Chen