PMID: 6986904Feb 19, 1980Paper

Synthesis of 8-14C-labeled O6-methyldeoxyguanosine and its deoxynucleotide copolymers

Biochemistry
P J AbbottD B Ludlum

Abstract

To study the nature and repair of the promutagenic DNA lesion O6-methylguanine, we have synthesized 8-14C-labeled O6-methyldeoxyguanosine triphosphate and investigated the kinetics of its incorporation into the synthetic copolymers poly(dC'm6dG) and poly(dT,m6dG). Deoxy[8-14C]guanosine was methylated with ethereal diazomethane and the products were separated by high-pressure liquid chromatography. O6-Methyldeoxy[14C]guanosine was converted to the 5'-monophosphate with carrot phosphotransferase and then to the 5'-triphosphate via the phosphorimidazolidate formed by the action of N,N'-carbonyldiimidazole. Although m6dGTP was a poor substrate for Escherichia coli DNA polymerase I, copolymers could be synthesized from dCTP or dTTP and m6dGTP with terminal deoxynucleotidyl transferase. The percent of m6dG in the polymer increased linearly as the percentage of m6dGTP in the polymerization mixture was increased to 20% of the total. The percent incorporation of m6dGTP into poly(dT,m6dG) was, however, higher than into poly(dC,m6dG). Good yields of both polymers were readily obtained. The stability of O6-methyldeoxyguanosine in poly(dT,m6dG) was found to be pH dependent, and the half-life has been measured at four different pH values.

References

Apr 1, 1975·Chemico-biological Interactions·S C Maitra, J V Frie
Dec 21, 1978·Biochimica Et Biophysica Acta·J R Mehta, D B Ludlum
Mar 28, 1969·Science·A Kornberg
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R Goth, M F Rajewsky
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D M Kirtikar, D A Goldthwait
May 10, 1973·Biochimica Et Biophysica Acta·L L Gerchman, D B Ludlum
Aug 1, 1970·Analytical Biochemistry·C L HarveyA L Nussbaum

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