Synthesis of 99mTc-HYNIC-interleukin-12, a new specific radiopharmaceutical for imaging T lymphocytes.

European Journal of Nuclear Medicine and Molecular Imaging
Alessio AnnovazziAlberto Signore

Abstract

Few radiopharmaceuticals have been described for the study of lymphocyte trafficking despite its high clinical relevance. The main difficulty resides in the identification of a suitable highly specific probe to target these cells. Interleukin-12 (IL12) is a heterodimeric cytokine which plays a key role in the development of Th(1) lymphocytes. The aims of the present study were to label IL12 with (99m)Tc, to evaluate its ability to bind to activated T lymphocytes in vitro and to study its biodistribution in normal mice and mice affected by autoimmune colitis. IL12 was derivatised with HYNIC-NHS and labelled with( 99m)Tc. An in vitro binding assay was performed on KIT225 cells, an IL12 receptor-positive cell line. (99m)Tc-IL12 biodistribution in normal mice was studied. Targeting experiments were performed in Balb/c mice injected with KIT225 cells and in mice with chemically induced chronic colitis. (99m)Tc-IL12 labelling efficiency ranged between 75% and 85%. Saturation binding analysis revealed a K (d) of 2.09 nM. Results of biodistribution studies showed a predominant hepatic route of excretion. A significant degree of uptake in the spleen and thymus was also observed. In mice injected with KIT225 cells, (99m)Tc-IL12-specific ...Continue Reading

References

Nov 1, 1995·The Journal of Experimental Medicine·M F NeurathW Strober
Mar 17, 1995·The Journal of Biological Chemistry·J J ZouU Gubler
Jan 1, 1996·Life Sciences·A S SternD H Presky
Nov 1, 1996·European Journal of Nuclear Medicine·P M van HagenE P Krenning
Mar 4, 1998·Arthritis and Rheumatism·J R Kalden, J Sieper
Oct 20, 1999·Cellular and Molecular Life Sciences : CMLS·L Adorini
Mar 4, 2003·Nuclear Medicine Communications·A SignoreP Pozzilli
May 30, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Paul J PockrosMichael F Debruin
Jun 28, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Jared A GollobMichael B Atkins
Sep 23, 2003·Nuclear Medicine and Biology·Kristin VerbekeAlfons Verbruggen
Jul 16, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Gheath AlatrashRonald M Bukowski
Nov 13, 2004·The New England Journal of Medicine·Peter J MannonUNKNOWN Anti-IL-12 Crohn's Disease Study Group

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Citations

Aug 13, 2011·Annals of Nuclear Medicine·Alberto Signore, Andor W J M Glaudemans
Jul 1, 2008·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Elizabeth J Akins, Purnima Dubey
Jan 11, 2012·Seminars in Vascular Surgery·Janneke L M BrugginkClark J Zeebregts
Feb 28, 2009·European Journal of Radiology·Robbert MeerwaldtClark J Zeebregts
Jan 9, 2008·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·M G van der VaartC J Zeebregts
Jan 1, 2013·Journal of Drug Delivery Science and Technology·N H KimO M Merkel
Mar 6, 2015·TheScientificWorldJournal·Chris Tsopelas
May 19, 2010·Expert Review of Clinical Immunology·Paul M Matthews, Robert Comley
Jun 26, 2020·Frontiers in Immunology·Claire E McCarthyHeather M Gibson
Apr 25, 2020·Frontiers in Physiology·Madeleine Iafrate, Gilbert O Fruhwirth
Apr 14, 2012·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Valentina Di GialleonardoErik F J De Vries

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