PMID: 2599762Sep 1, 1989Paper

Synthesis of a biotinylated, iodinatable, and photoactivatable probe for angiotensin receptors

International Journal of Peptide and Protein Research
R SeyerB Castro


We propose here a biotinyl-aminohexanoyl-[Ala1, Phe(4N3)8]angiotensin II analog as a radioiodinatable and photoactivatable probe for covalent labeling, detection and isolation of angiotensin receptors. A combination of solid phase and minimum-protection segment-coupling strategy using hexafluorophosphate of (benzotriazol-1-yloxy)tris(dimethylamino)phosphonium (BOP) as a coupling reagent is proposed for the synthesis of this probe. Optimized yields were obtained by HPLC monitoring of all reactions. A complete n.m.r. study suggests an extended conformation of this molecule, allowing a simultaneous recognition of receptor and avidin. The probe binds with high affinity (Kd = 2 nM) to angiotensin II receptors from rat liver membranes.


Jan 5, 1977·Journal of the American Chemical Society·M Bodanszky, D T Fagan
Jan 1, 1986·International Journal of Peptide and Protein Research·C Garbay-JaureguiberryB P Roques
Mar 25, 1986·Biochemistry·K HofmannP K Mishra
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A AumelasF M Bumpus
Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J Houmard, G R Drapeau
Nov 1, 1984·International Journal of Peptide and Protein Research·S LavielleA Marquet
Jan 1, 1984·Journal of Receptor Research·G GuillemetteS Jard
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·I SenR L Soffer
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·F M FinnK Hofmann
Jul 1, 1983·International Journal of Peptide and Protein Research·A S AcharyaJ M Manning

❮ Previous
Next ❯


Dec 1, 1994·International Journal of Peptide and Protein Research·M J MacielagI Depoortere
May 1, 1990·International Journal of Peptide and Protein Research·R SeyerB Castro
Mar 1, 1992·The International Journal of Biochemistry·R SeyerA Aumelas

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.