Oct 1, 1976

Synthesis of a liver enzyme in hybrid cells

Journal of Cell Science
V G Riddle, H Harris

Abstract

Rat hepatoma cells were fused with cells of an established mouse lymphoma line, with normal diploid mouse macrophages, lymphocytes and fibroblasts and with normal diploid rat macrophages and lymphocytes. The liver-specific enzyme tyrosine aminotransferase was produced by almost all the hybrid cells, but usually at a lower level than in the parental hepatoma cells. Most of the hybrids also showed increased levels of this enzyme after exposure to dexamethasone. In the rat x mouse hybrids, the electrophoretic mobility of the enzyme indicated that only the rat hepatoma enzyme was produced. The findings are difficult to explain in terms of simple models involving a single diffusible repressor or activator of tyrosine aminotransferase synthesis.

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Mentioned in this Paper

SDS-PAGE
Liver Carcinoma
Lymphoid Cells
Tyrosine Aminotransferase
Spectrophotometry
Macrophage
Hybrid Cells
Enzyme Induction
Ectromelia, Infectious
Karyotype Determination Procedure

About this Paper

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