Synthesis of a monolithic, micro-immobilised enzyme reactor via click-chemistry

Analytical and Bioanalytical Chemistry
Bekir ÇelebiAli Tuncel

Abstract

An immobilised enzyme reactor (IMER) in the form of capillary monolith was developed for a micro-liquid chromatography system. The plain monolith was obtained by in situ thermal copolymerisation of glycidyl methacrylate and ethylene dimethacrylate in a fused silica capillary (200 × 0.53 mm ID) by using n-propanol/1,4-butanediol as porogen. The enzyme, α-chymotrypsin (CT), was covalently attached onto the monolith via triazole ring formation by click-chemistry. For this purpose, the monolithic support was treated with sodium azide and reacted with the alkyne carrying enzyme derivative. CT was covalently linked to the monolith by triazole-ring formation. The activity behaviour of monolithic IMER was investigated in a micro-liquid chromatography system by using benzoyl-L-tyrosine ethyl ester (BTEE) as synthetic substrate. The effects of mobile-phase flow rate and substrate feed concentration on the final BTEE conversion were investigated under steady-state conditions. In the case of monolithic IMER, the final substrate conversion increased with increasing feed flow rate and increasing substrate feed concentration. Unusual behaviour was explained by the presence of convective diffusion in the macropores of monolith. The results ind...Continue Reading

References

Jul 26, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Quanzhou LuoHanfa Zou
Sep 6, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Martina VodopivecAles Strancar
Mar 31, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anna Maria Girelli, Enrico Mattei
Jul 15, 2005·Analytical Chemistry·Maria T DulayRichard N Zare
Oct 18, 2005·Journal of Separation Science·Jana KrenkováFrantisek Foret
Sep 18, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·C DelattreM A Vijayalakshmi
Sep 28, 2007·Analytical and Bioanalytical Chemistry·E C A StigterW P van Bennekom
Dec 4, 2008·Journal of Separation Science·Michael D SlaterFrantisek Svec
Mar 21, 2009·Journal of Separation Science·Caterina TemporiniGabriella Massolini
Jan 29, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·E A PonomarevaT B Tennikova
Nov 17, 2010·Analytical and Bioanalytical Chemistry·Xiangli SunYukui Zhang
Aug 10, 2011·The FEBS Journal·Hu ZhouDaniel Figeys

❮ Previous
Next ❯

Citations

Mar 8, 2016·Journal of Chromatography. a·Kinga MellerBogusław Buszewski
Sep 7, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kinga MellerBogusław Buszewski
Mar 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sarah Alharthi, Ziad El Rassi
Apr 24, 2013·Journal of Separation Science·Audrey MarechalClaire Demesmay
Jan 17, 2013·Chemical Communications : Chem Comm·Minghuo WuXing-Fang Li
Feb 14, 2013·ACS Medicinal Chemistry Letters·Cristina PeruzzottiDaniele Passarella
Nov 7, 2014·Analytical Chemistry·Frantisek Svec, Yongqin Lv

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.