Synthesis of a novel L-methyl-methionine-ICG-Der-02 fluorescent probe for in vivo near infrared imaging of tumors.

Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging
Didel M MahoungaYueqing Gu

Abstract

A novel near infrared fluorescent probe, L-methyl-methionine (Met)-ICG-Der-02, was synthesized and characterized for in vivo imaging of tumors and early diagnosis of cancers. Met was conjugated with ICG-Der-02 dye through the amide bond function by ethyl-3-(3-dimethyllaminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide catalysis chemistry. Met-ICG-Der-02 probe uptake was evaluated on PC3, MDA-MB-231, and human embryonic lung fibroblast cell lines. The dynamics of Met-ICG-Der-02 was investigated in athymic nude mice prior to evaluation of the probe targeting capability in prostate and breast cancer models. Met-ICG-Der-02 was successfully synthesized. Cell experiments demonstrated excellent cellular uptake of Met-ICG-Der-02 on cancer cell lines without cytotoxicity. Optical imaging showed a distinguishable fluorescence signal in the tumor area at 2 h while maximal tumor-to-normal tissue contrast ratio was at 12 h Met-ICG-Der-02 post-injection. Additionally, dynamic study of the probe indicated intestinal and liver-kidney clearance pathways. Met-ICG-Der-02 probe is a promising optical imaging agent for tumor diagnosis, especially in their early stage.

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Citations

Jul 10, 2014·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Haiyan ChenYueqing Gu
Feb 14, 2014·Contrast Media & Molecular Imaging·Haiyan ChenYueqing Gu
Apr 17, 2015·Drug Testing and Analysis·Luwei ZhouYanjiang Qiao
May 6, 2015·Oncotarget·Siwen LiYueqing Gu
Feb 5, 2021·Biomaterials Science·Jie LiuJun Wu
Dec 17, 2018·International Journal of Biological Macromolecules·Ayman Y WaddadThirumala Govender

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