PMID: 39626Jul 4, 1979

Synthesis of adenosine 3',5'-monophosphate by guanylate cyclase, a new pathway for its formation

Biochimica Et Biophysica Acta
C K MittalFerid Murad


The 105 000 X g gupernatant fractions from homogenates of various rat tissues catalyzed the formation of both cyclic GMP and cyclic AMP from GTP and ATP, respectively. Generally cyclic AMP formation with crude or purified preparations of soluble guanylate cyclase was only observed when enzyme activity was increased with sodium azide, sodium nitroprusside, N-methyl-N'-nitro-N-nitrosoguanidine, sodium nitrite, nitric oxide gas, hydroxyl radical and sodium arachidonate. Sodium fluoride did not alter the formation of either cyclic nucleotide. After chromatography of supernatant preparations on Sephadex G-200 columns or polyacrylamide gel electrophoresis, the formation of cyclic AMP and cyclic GMP was catalyzed by similar fractions. These studies indicate that the properties of guanylate cyclase are altered with activation. Since the synthesis of cyclic AMP and cyclic GMP reported in this study appears to be catalyzed by the same protein, one of the properties of activated guanylate cyclase is its ability to catalyze the formation of cyclic AMP from ATP. The properties of this newly described pathway for cyclic AMP formation are quite different from those previously described for adenylate cyclase preparations. The physiological sig...Continue Reading


Sep 29, 2000·Pharmacology & Therapeutics·F SchwedeB Jastorff
Jun 17, 2009·Biochemistry·Emily R DerbyshireMichael A Marletta
Jun 12, 2010·Cardiology in Review·Vaughn E NossamanPhilip J Kadowitz
Jun 15, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Adeleke M BadejoPhilip J Kadowitz
Nov 10, 2006·The New England Journal of Medicine·Ferid Murad
Jan 24, 2007·Physiological Reviews·Pál PacherLucas Liaudet
Oct 11, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ahmed M HamadAlan J Knox


Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W P ArnoldFerid Murad
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H Hidaka, T Asano
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C K Mittal, Ferid Murad
Mar 1, 1975·Metabolism: Clinical and Experimental·H Kimura, Ferid Murad
Sep 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·A G Gilman
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J M BraughlerFerid Murad

Related Concepts

Biochemical Pathway
Sodium Nitroprusside
Sephadex G 200
Cyclic GMP Measurement (Lab Test)
Cyclic AMP

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.