PMID: 39626Jul 4, 1979

Synthesis of adenosine 3',5'-monophosphate by guanylate cyclase, a new pathway for its formation

Biochimica Et Biophysica Acta
C K MittalFerid Murad

Abstract

The 105 000 X g gupernatant fractions from homogenates of various rat tissues catalyzed the formation of both cyclic GMP and cyclic AMP from GTP and ATP, respectively. Generally cyclic AMP formation with crude or purified preparations of soluble guanylate cyclase was only observed when enzyme activity was increased with sodium azide, sodium nitroprusside, N-methyl-N'-nitro-N-nitrosoguanidine, sodium nitrite, nitric oxide gas, hydroxyl radical and sodium arachidonate. Sodium fluoride did not alter the formation of either cyclic nucleotide. After chromatography of supernatant preparations on Sephadex G-200 columns or polyacrylamide gel electrophoresis, the formation of cyclic AMP and cyclic GMP was catalyzed by similar fractions. These studies indicate that the properties of guanylate cyclase are altered with activation. Since the synthesis of cyclic AMP and cyclic GMP reported in this study appears to be catalyzed by the same protein, one of the properties of activated guanylate cyclase is its ability to catalyze the formation of cyclic AMP from ATP. The properties of this newly described pathway for cyclic AMP formation are quite different from those previously described for adenylate cyclase preparations. The physiological sig...Continue Reading

References

Sep 29, 2000·Pharmacology & Therapeutics·F SchwedeB Jastorff
Jun 17, 2009·Biochemistry·Emily R DerbyshireMichael A Marletta
Jun 12, 2010·Cardiology in Review·Vaughn E NossamanPhilip J Kadowitz
Jun 15, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Adeleke M BadejoPhilip J Kadowitz
Nov 10, 2006·The New England Journal of Medicine·Ferid Murad
Jan 24, 2007·Physiological Reviews·Pál PacherLucas Liaudet
Oct 11, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ahmed M HamadAlan J Knox

Citations

Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W P ArnoldFerid Murad
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H Hidaka, T Asano
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C K Mittal, Ferid Murad
Mar 1, 1975·Metabolism: Clinical and Experimental·H Kimura, Ferid Murad
Sep 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·A G Gilman
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J M BraughlerFerid Murad

Related Concepts

Biochemical Pathway
Sodium Nitroprusside
Sephadex G 200
Methylnitronitrosoguanidine
Adenosine
Lung
Cyclic GMP Measurement (Lab Test)
Chromatography
Cyclic AMP
Kidney

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