Synthesis of an enzyme-like imprinted polymer with the substrate as the template, and its catalytic properties under aqueous conditions

Chemistry : a European Journal
Zhiyong ChengYuanzong Li

Abstract

Transition state analogues (TSAs) have long been regarded as ideal templates for the preparation of catalytically active synthetic imprinted polymers. In the current work, however, a new type of molecularly imprinted polymer (MIP) was synthesized with the substrate (homovanillic acid, HVA) as the template and hemin introduced as the catalytic center, with the use of plural functional monomers to prepare the active sites. The MIP successfully mimicked natural peroxidase, suggesting that it may not be imperative to employ a TSA as the template when preparing enzyme-like imprinted polymers and that the imprinted polymer matrix provided an advantageous microenvironment around the catalytic center (hemin), essentially similar to that supplied by apo-proteins in natural enzymes. Significantly, by taking advantage of the special structure of hemin and multiple-site interactions provided by several functional monomers, the intrinsic difficulties for MIPs in recognizing template molecules in polar solutions were overcome. The newly developed polymer showed considerable recognizing ability toward HVA, catalytic activity, substrate specificity and also stability, which are the merits lacked by the natural peroxidase. Meanwhile, the ease o...Continue Reading

Citations

Apr 5, 2014·Journal of Molecular Recognition : JMR·Michael J WhitcombeIan A Nicholls
May 17, 2007·Analytical and Bioanalytical Chemistry·Daniel S Janiak, Peter Kofinas
Sep 29, 2007·Analytical and Bioanalytical Chemistry·Wilney de Jesus Rodrigues SantosLauro Tatsuo Kubota
Jul 8, 2008·Chemistry : a European Journal·Davide CarboniMarina Resmini
Jun 25, 2005·Chemistry : a European Journal·Estelle BurriKay Severin
May 14, 2020·Sensors·Aysu Yarman, Frieder W Scheller
Mar 10, 2016·Nanoscale·Maria HitrikDaniel Mandler
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Apr 13, 2018·Analytical Chemistry·Qiusha LiZheng-Zhi Yin
Jan 20, 2021·ACS Biomaterials Science & Engineering·Xiaoqiang WangFang Huang

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