PMID: 775491May 1, 1976

Synthesis of an R plasmid protein associated with tetracycline resistance is negatively regulated

Proceedings of the National Academy of Sciences of the United States of America
H L YangS B Levy

Abstract

Synthesis of proteins encoded by the R222 plasmid was observed in a DNA-directed cell-free system and the products were compared to those plasmid proteins synthesized in Escherichia coli minicells. A greater number of plasmid-specified proteins was detected in the in vitro system than in the minicell, suggesting the presence of control factors for plasmid gene expression in the minicell. Synthesis of a newly detected plasmid protein (TET protein) is induced by tetracycline in minicells containing tetracycline-resistant plasmids, including R222, and this induced synthesis correlates with induced host resistance to the drug. This TET protein was synthesized in vitro from R222 DNA in the absence of tetracycline, indicating that no positive regulatory role for tetracycline is required for the protein's synthesis. TET proteon synthesis was inhibited in vitro when cell-free extracts prepared from cells containing the R222 plasmid were used.

References

Feb 4, 1974·Biochemical and Biophysical Research Communications·H L Yang, G Zubay
Jan 1, 1973·Annual Review of Genetics·G Zubay
Feb 27, 1974·Biochemical and Biophysical Research Communications·S B Levy, L McMurry
Sep 15, 1973·Journal of Molecular Biology·F W Studier
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Citations

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M A Savageau
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H L YangG Zubay
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L McMurryS B Levy
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J L ErionN Brot
Jan 25, 1983·Nucleic Acids Research·W Hillen, K Schollmeier
May 27, 1986·Nucleic Acids Research·A WissmannW Hillen
Aug 25, 1989·Nucleic Acids Research·K Tovar, W Hillen
May 1, 1977·Antimicrobial Agents and Chemotherapy·V L Clark, F E Young
Dec 1, 1978·Antimicrobial Agents and Chemotherapy·A A WeissT G Kinscherf
Mar 13, 2016·BMC Microbiology·Thea S B MøllerJohn E Olsen
Mar 1, 1980·Plasmid·B MendezS B Levy
Mar 1, 1997·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·V D Magalhães, B A Castilho
Aug 20, 1985·Journal of Molecular Biology·D W Daniels, K P Bertrand
Sep 6, 2013·Microbiology and Molecular Biology Reviews : MMBR·Leslie Cuthbertson, Justin R Nodwell
Jan 11, 2019·Microbiology·Sylvia OetermannAlexander Steinbüchel
Aug 5, 2017·Scientific Reports·Cordelia P N RampleyWei E Huang

Related Concepts

Bacterial Proteins
Antibiotic Resistance, Microbial
Alkalescens-Dispar Group
Cistron
Genes, Regulator
R Factors
RNA, Viral
Topicycline
Transcription, Genetic
Protein Biosynthesis

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