Synthesis of biocompatible, BSA capped fluorescent CaCO3 pre-nucleation nanoclusters for cell imaging applications.

Journal of Materials Chemistry. B, Materials for Biology and Medicine
Shivesh SabbarwalManoj Kumar

Abstract

Novel, photostable, multicolour fluorescent, highly biocompatible, water soluble, BSA capped pre-nucleation CaCO3 nanoclusters (FCPN) (∼1.3 nm) are developed using a facile biomineralization process. M. oleifera leaf extract and BSA protein are used as sources of ascorbic acid and capping agent, respectively. The developed FCPN shows fluorescence in the blue, green, and yellow/red region with an average life time of 1.05, 6.23 and 30.60 ns, respectively. The MALDI-MS measurements reveal that these nanoclusters are 16, 50, 73, 222 and 936 molecules big. These FCPN, when incubated (up to 7 days) with MG-63 cells, demonstrate an increase in cell viability percentage with time period as compared to their control samples. Furthermore, these incubated cells were investigated using confocal microscopy to estimate the FCPN diffusion penetration depth using CTCF analysis. It has been observed that blue and green emitting FCPN penetrated 6 μm, whereas red emitting FCPN traversed only 4 μm. The relative quantum yield (Rhodamine 6G = 0.92) of FCPN for green emission was found to be 0.0175 in water. The prepared nanoclusters displayed four months shelf-life. These FCPN were prepared using an environmentally benign, inexpensive, green synthe...Continue Reading

References

Dec 4, 2003·Mutation Research·Michael P Waalkes
Mar 22, 2006·Chemical Communications : Chem Comm·David J LewisZoe Pikramenou
Nov 16, 2006·Annual Review of Physical Chemistry·Jie ZhengRobert M Dickson
Mar 21, 2007·Analytical Biochemistry·Yang WuTione Buranda
Nov 28, 2007·Optics Letters·M DahanS Weiss
Feb 23, 2008·Chemical Communications : Chem Comm·Li Shang, Shaojun Dong
Dec 20, 2008·Science·Denis GebauerHelmut Cölfen
Jan 7, 2009·Journal of the American Chemical Society·Jianping XieJackie Y Ying
May 29, 2009·Journal of Nanobiotechnology·Helena MontónMònica Roldán
Apr 20, 2010·Advanced Materials·Hangxun Xu, Kenneth S Suslick
Jun 17, 2010·Nano Letters·Zhikun Wu, Rongchao Jin
Oct 1, 2010·Nanoscale·Paulrajpillai Lourdu XavierThalappil Pradeep
Jun 21, 2011·International Journal of Molecular Sciences·Guang-Jun HeYong-Bin Yan
Dec 22, 2011·Nature Communications·Raffaella DemichelisDenis Gebauer
Mar 31, 2012·Biomaterials·Xiao-Dong ZhangSai-Jun Fan
Nov 28, 2012·Journal of Environmental Management·Miran YooJung-Ho Wee
Aug 16, 2013·The Analyst·Quanwen LiuDa-Peng Yang
Sep 17, 2013·Chemical Communications : Chem Comm·Yong YuJianping Xie
Dec 24, 2013·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Jian ZhangGangli Wang
Jan 22, 2014·ACS Applied Materials & Interfaces·V VenkateshSandeep Verma
Feb 28, 2014·ACS Applied Materials & Interfaces·Rama GhoshArun Chattopadhyay
Mar 5, 2014·Chemical Reviews·Kyle P CarterAmy E Palmer
Jun 8, 2014·The Journal of Surgical Research·George P LiaoCharles S Cox
Jul 6, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gabriel Paës
Dec 4, 2014·Advances in Colloid and Interface Science·Ajay Singh KarakotiSanjay Singh
Apr 8, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Shanshan GuoWei Gu
Jul 15, 2015·Journal of Orthopaedics and Traumatology : Official Journal of the Italian Society of Orthopaedics and Traumatology·Olga CharyevaKatrin Susanne Lips
Sep 20, 2012·The Journal of Physical Chemistry Letters·Wataru KurashigeYuichi Negishi
Apr 6, 2016·Scientific Reports·Puskal KunwarJuha Toivonen
May 11, 2016·Chemical Communications : Chem Comm·Shengtong SunHelmut Cölfen
Jul 19, 2016·International Journal of Cancer. Journal International Du Cancer·Mara SaccomanoJoanna Napp
Sep 23, 2016·Journal of the American Chemical Society·Qi-Wei ZhangHe Tian
Nov 20, 2016·Nature Communications·David C GreenFiona C Meldrum
Jan 10, 2017·Scientific Reports·Saravanan GovindarajuKyusik Yun
Dec 28, 2018·The Science of the Total Environment·Hannah Y H ChangJ C Liu
Feb 14, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Seyed Nasser OstadJafar Mahmoudian

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Methods Mentioned

BETA
ELISA
fluorescence imaging
transmission electron microscopy
X-ray
fluorescence studies
electron microscopy
fluorescence microscopy

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