Synthesis of bioinspired collagen/alginate/fibrin based hydrogels for soft tissue engineering

Materials Science & Engineering. C, Materials for Biological Applications
G MontalbanoA M Ferreira

Abstract

Hydrogels based on natural polymers offer a range of properties to mimic the native extracellular matrix, and provide microenvironments to preserve cellular function and encourage tissue formation. A tri-component hydrogel using collagen, alginate and fibrin (CAF) was developed and investigated at three collagen concentrations for application as a functional extracellular matrix analogue. Physical-chemical characterization of CAF hydrogels demonstrated a thermo-responsive crosslinking capacity at physiological conditions with stiffness similar to native soft tissues. CAF hydrogels were also assessed for cytocompatibility using L929 murine fibroblasts, pancreatic MIN6 β-cells and human mesenchymal stem cells (hMSCs); and demonstrated good cell viability, proliferation and metabolic activity after 7 days of in vitro culture. CAF hydrogels, especially with 2.5% w/v collagen, increased alkaline phosphatase production in hMSCs indicating potential for the promotion of osteogenic activity. Moreover, CAF hydrogels also increased metabolic activity of MIN6 β-cells and promoted the reconstitution of spherical pseudoislets with sizes ranging between 50 and 150 μm at day 7, demonstrating potential in diabetic therapeutic applications.

Citations

Apr 26, 2019·Journal of Cellular Biochemistry·Neda KhatamiReza Rahbarghazi
Dec 14, 2018·Biofabrication·Ricardo da Conceicao RibeiroKenneth Dalgarno
Aug 21, 2020·Macromolecular Rapid Communications·Jun Kit WangChor Yong Tay
Jul 23, 2020·Frontiers in Bioengineering and Biotechnology·Annachiara ScalzonePiergiorgio Gentile
Apr 10, 2019·Polymers·Sanjay TiwariPratap Bahadur
Oct 22, 2020·International Journal of Molecular Sciences·Antonia MancusoDonatella Paolino
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Zahra Hajian ForoushaniMehrab Mehrvar
Sep 20, 2020·Acta Biomaterialia·Bruna A G de MeloMarimelia A Porcionatto
Feb 14, 2021·Materials·Magdalena B ŁabowskaIzabela Michalak
Dec 17, 2020·Materials·Ruxandra-Elena Geanaliu-Nicolae, Ecaterina Andronescu
Mar 4, 2020·Carbohydrate Polymers·Henna CassimjeeViness Pillay
Apr 4, 2021·International Journal of Molecular Sciences·Soojin LeeYoungmee Jung
Apr 24, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Gaurav KulkarniSoumen Das
Jun 29, 2021·Frontiers in Bioengineering and Biotechnology·Tian ZhouXiaomeng Zhang
Sep 7, 2021·Journal of Biomedical Materials Research. Part a·Likitha SomasekharKunal Mitra

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