Synthesis of cadmium, lead and copper alginate nanobeads as immunosensing probes for the detection of AFP, CEA and PSA

Biosensors & Bioelectronics
Zifeng WangZhanfang Ma

Abstract

A double-water-in-oil-emulsion procedure was designed to synthesize cadmium, lead and copper alginate nanobeads less than 200nm diameter under mild conditions. The cadmium, lead and copper alginate nanobeads can be activated to immobilize biomacromolecules and can directly produce distinctive electrochemical signals. Using the novel alginate nanobeads labeled with antibodies as electrochemical probes, a sandwich-type immunosensor was constructed using AFP, CEA and PSA as model analytes. This proposed immunosensor shows wide linear range with detection limits of 0.01, 0.0086 and 0.0075ngmL(-1) for AFP, CEA and PSA, respectively. Analysis of clinical serum samples using this immunosensor was well consistent with the data determined by the enzyme-linked immunosorbent assay (ELISA). It suggested that the alginate nanobeads electrochemical probes could be generally extended to other multiple analytes detection.

References

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Citations

Jun 2, 2015·Expert Review of Molecular Diagnostics·Zhanfang Ma, Na Liu
Apr 28, 2017·Sensors·Paloma Yáñez-SedeñoJosé M Pingarrón
Apr 26, 2018·Reviews on Environmental Health·Moussa BakayokoEl Moctar Ismaila
May 4, 2018·Expert Review of Molecular Diagnostics·Shuang Yin, Zhanfang Ma
Feb 6, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Sayeh Dowlatshahi, Mohammad J Abdekhodaie
Aug 25, 2021·Analytical Chemistry·Shaohua GongBo Tang

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