Synthesis of collagen by bovine chondrocytes cultured in alginate; posttranslational modifications and cell-matrix interaction

Experimental Cell Research
B BeekmanJ M TeKoppele

Abstract

The extracellular matrix synthesized by articular chondrocytes cultured in alginate beads was investigated. Collagen levels increased sigmoidally with time and remained constant after 2 weeks of culture. The presence of cartilage-specific type II collagen was confirmed immunohistochemically. Predominantly type II collagen was present in the alginate bead, as reflected by the unique extent of lysyl hydroxylation, glycosylation, and pyridinoline crosslink formation measured. Collagen crosslinks, predominantly hydroxylysylpyridinoline (> 93%), were observed after 7 to 11 days of culture and their formation was effectively blocked by beta-aminopropionitrile (BAPN). Unexpectedly, BAPN treatment resulted in a 100% increase of collagen levels, without influencing cell proliferation and proteoglycan levels. In control cultures 90% of the synthesized collagen was retained in the cell-associated matrix, while in BAPN-treated cultures half of the collagen was found in the interterritorial matrix compartment further removed from the cells. This suggests that impaired crosslinking of collagen interferes with pericellular collagen deposition, causing upregulation of collagen synthesis by impaired cell-matrix interactions. Integrins are likel...Continue Reading

Citations

Apr 5, 2001·Journal of Biomedical Materials Research·S C ChangL J Bonassar
Sep 5, 2002·Journal of Biomedical Materials Research·Stephen D WaldmanRita A Kandel
Jun 12, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Stephen D WaldmanRita A Kandel
Oct 31, 2008·Journal of Tissue Engineering and Regenerative Medicine·Farhad ForoughiRobert A Brown
Mar 23, 2011·Arthritis and Rheumatism·Naoshi FukuiShigeto Tohma
Nov 27, 1998·Journal of Cellular Biochemistry·J RiesleG Vunjak-Novakovic
Jun 15, 2006·Tissue Engineering·Daniel L CohenLawrence J Bonassar
Jan 25, 2003·Tissue Engineering·Marcy WongDaniel Studer
Jan 23, 2009·Journal of Tissue Engineering and Regenerative Medicine·Y M Bastiaansen-JenniskensJ DeGroot
Mar 13, 2008·Tissue Engineering. Part a·Stephen D WaldmanStephen C Pang
Feb 10, 2010·Tissue Engineering. Part a·Vlada StamenkovićShawn Patrick Grogan
Jun 29, 2010·Journal of Materials Science. Materials in Medicine·Lin SangXudong Li
Jan 31, 2009·Arthritis and Rheumatism·Christine Lyn Le MaitreJudith Alison Hoyland
Aug 11, 2011·Tissue Engineering. Part B, Reviews·David A BicharaMark A Randolph
Aug 13, 2011·Journal of Materials Science. Materials in Medicine·Mohammad Kazem GhahramanpoorMohamadreza Baghaban Eslaminejad
Jan 21, 2014·Journal of Biomedical Materials Research. Part a·Dan DengRobert A Brown
Mar 1, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ju Young ParkDong-Woo Cho
May 15, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·E AlsbergD J Mooney
Feb 11, 1999·Advances in Dental Research·J M TeKoppeleR A Bank
Mar 31, 2009·Tissue Engineering. Part C, Methods·Mihye KimYoung Ha Kim
Mar 5, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eleftherios A MakrisKyriacos A Athanasiou
Sep 10, 2003·Plastic and Reconstructive Surgery·Sophia C N ChangLawrence J Bonassar
Jan 1, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Stephen D WaldmanRita A Kandel
Oct 18, 2001·Clinical Orthopaedics and Related Research·S P ScullyW Qi
Oct 7, 2008·The Journal of Biological Chemistry·Phimon AtsawasuwanMitsuo Yamauchi
Oct 3, 2017·Nature Materials·Hong-Pyo LeeOvijit Chaudhuri
Jan 17, 2017·Tissue Engineering and Regenerative Medicine·Linyi CaiJing Xie
Sep 9, 2010·Tissue Engineering. Part C, Methods·Daniel L CohenLawrence J Bonassar
Jun 12, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M WongE Hunziker
May 2, 2003·The Journal of Bone and Joint Surgery. American Volume·Stephen D WaldmanRita A Kandel
Sep 21, 2020·Progress in Retinal and Eye Research·Sayon Roy, Dongjoon Kim

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