PMID: 3764345Oct 1, 1986Paper

Synthesis of complement components C5, C6, C7, C8 and C9 in vitro by human monocytes and assembly of the terminal complement complex

Scandinavian Journal of Immunology
G HetlandT Eskeland

Abstract

Monocytes cultured under serum-free conditions secreted protein which bound covalently and non-covalently to agarose beads, an activator of the alternative pathway of complement. There was a significantly binding of monoclonal anti-C3c antibodies, polyclonal anti-C5, anti-C6, anti-C7, anti-C8, and anti-C9 antibodies, and of a monoclonal antibody against a neoantigen of polymerized C9 to agarose beads incubated with the monocytes for 24, 48, 72 or 96 h. From these results, we conclude that monocytes produce C5, C6, C7, C8 and C9 that assemble as the terminal complement complex on the surface of the agarose beads. Activation by agarose of the alternative pathway with generation of particle bound C3 and C5 convertases is a prerequisite for the subsequent formation of the terminal complement complex. Whether SC5b-9 or the membrane attack of complement (C5b-9) is formed on the beads will be examined.

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Citations

Jul 5, 1991·Journal of Immunological Methods·G HetlandG Størvold
Dec 1, 1996·Molecular Immunology·C S LetsonJ M Sodetz
Jul 1, 1995·Molecular Medicine Today·P A AndrewsS H Sacks
Jul 11, 2000·Clinical and Experimental Immunology·H LangeggenF Tedesco
Jun 1, 1991·Scandinavian Journal of Immunology·E Johnson, G Hetland
Feb 3, 2007·Molecular Immunology·Krisztina K TimárSyed S Asghar
Mar 2, 2017·Clinical and Experimental Immunology·R LubbersL A Trouw
Jun 1, 1987·Scandinavian Journal of Immunology·H B PettersenG Hetland
Oct 1, 1988·Scandinavian Journal of Immunology·H B PettersenP Garred
Jan 1, 1989·Scandinavian Journal of Immunology·G HetlandE Johnson
Jan 1, 1990·Scandinavian Journal of Immunology·H B PettersenS S Osen
Nov 1, 1990·Scandinavian Journal of Immunology·P GarredG Stoervold
May 1, 1988·Scandinavian Journal of Immunology·E Johnson, G Hetland
Sep 1, 1996·European Journal of Immunology·M A NaughtonM Botto
May 1, 1988·Scandinavian Journal of Clinical and Laboratory Investigation·E Johnson, G Hetland
Jan 1, 1989·Autoimmunity·N ScoldingA Compston
Jun 1, 1987·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology·G HetlandT Eskeland
Mar 10, 2018·Frontiers in Immunology·Katharina BrökerChristian M Karsten
Nov 1, 1993·European Journal of Haematology·R Seljelid, T Eskeland

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