Synthesis of complementary RNA by RNA-dependent RNA polymerases in plant extracts is independent of an RNA primer

Functional Plant Biology : FPB
Lei WangMing-Bo Wang

Abstract

RNA-dependent RNA polymerase (RDR) activities were readily detected in extracts from cauliflower and broccoli florets, Arabidopsis thaliana (L.) Heynh callus tissue and broccoli nuclei. The synthesis of complementary RNA (cRNA) was independent of a RNA primer, whether or not the primer contained a 3' terminal 2'-O-methyl group or was phosphorylated at the 5' terminus. cRNA synthesis in plant extracts was not affected by loss-of-function mutations in the DICER-LIKE (DCL) proteins DCL2, DCL3, and DCL4, indicating that RDRs function independently of these DCL proteins. A loss-of-function mutation in RDR1, RDR2 or RDR6 did not significantly reduce the amount of cRNA synthesis. This indicates that these RDRs did not account for the bulk RDR activities in plant extracts, and suggest that either the individual RDRs each contribute a fraction of polymerase activity or another RDR(s) is predominant in the plant extract.

References

Aug 13, 2002·Molecular Genetics and Genomics : MGG·Y Han, Donald Grierson
Jan 7, 2003·Genes & Development·Guiliang TangPhillip D Zamore
Aug 28, 2003·The EMBO Journal·Christophe HimberOlivier Voinnet
Mar 17, 2004·PLoS Biology·Zhixin XieJames C Carrington
Apr 1, 2004·The Plant Journal : for Cell and Molecular Biology·Ulrike VogtMichael Wassenegger
Apr 27, 2005·Cell·Edwards AllenJames C Carrington
Jul 16, 2005·Plant Molecular Biology·Birgit Otzen Petersen, Merete Albrechtsen
Sep 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·H Alexander EbhardtPeter J Unrau
Feb 14, 2006·Trends in Plant Science·Michael Wassenegger, Gabi Krczal
Oct 14, 2006·EMBO Reports·Adriana F FusaroPeter M Waterhouse
Dec 13, 2006·Science·Titia SijenRonald H A Plasterk
Dec 8, 2007·The Journal of Biological Chemistry·Julien Curaba, Xuemei Chen

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