PMID: 3894020Jul 1, 1985

Synthesis of Escherichia coli carbamoylphosphate synthetase initiates at a UUG codon

European Journal of Biochemistry
G WeyensA Piérard


The ribosome binding region of the messenger RNA for the Escherichia coli carA gene contains two adjacent putative translational start codons, UUG and AUU, both of them unusual. By Edman degradation and mass spectrometry of purified carA protein, we show that only UUG is used in vivo. Translation initiation at UUG in carA appears about half as efficient as at AUG in lacZ.


Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D BelinR H Epstein
Jul 1, 1977·Journal of Molecular Biology·D Kennell, H Riezman
Jun 25, 1975·Journal of Molecular Biology·J G FilesJ H Miller
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
Aug 1, 1980·Journal of Bacteriology·D GigotA Piérard
Jan 1, 1981·Annual Review of Microbiology·L GoldG Stormo
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J BouvierP Stragier
Aug 1, 1980·Journal of Bacteriology·M CrabeelN Glansdorff

Related Concepts

Alkalescens-Dispar Group
Ribosome Binding
Codon, Initiator
Sense Codon
Mass Spectrometry
Codon Genus
Peptide Fragments
Codon (Nucleotide Sequence)
Peptide Chain Initiation, Translational

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