PMID: 8972847Dec 1, 1996Paper

Synthesis of full-length oligonucleotides: cleavage of apurinic molecules on a novel support

Nucleic Acids Research
M KwiatkowskiUlf Landegren

Abstract

The synthesis of oligodeoxynucleotides is marred by several problems that contribute to the formation of defective molecules. This in turn seriously limits the usefulness of such reagents in DNA diagnostics, molecular cloning, DNA structural analysis and in antisense therapy. In particular, depurination reactions during the cyclical steps of synthesis lead to strand scission during cleavage of the completed molecules from the support. Here we present a remedy to this problem: a novel disiloxyl linkage that connects oligonucleotides to the support withstands reaction conditions that allow the removal of the 5' parts of any depurinated molecules. This ensures that all molecules that preserve the 5' protecting group when cleaved from the support will have both correct 3'- and 5'-ends. We demonstrate the application of the support for synthesis of padlock probe molecules.

References

Aug 26, 1986·Nucleic Acids Research·W H McClainJ Schneider
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Citations

Dec 24, 2003·Trends in Biotechnology·Weiguo Cao
Feb 13, 2001·Current Opinion in Biotechnology·J BanérU Landegren
Jun 28, 2000·Nucleic Acids Research·D O AntsonM Nilsson
Jan 12, 2008·Nucleic Acids Research·Marcus Gry BjörklundJoakim Lundeberg
Sep 18, 2002·Nucleic Acids Research·Ji-Yen ChengKonan Peck
Apr 30, 1998·Annals of Medicine·U Landegren, M Nilsson
Jun 18, 2015·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Gholam Reza ZamaniMahdi Zamani
Feb 5, 2011·Chemistry : a European Journal·Diana C KnappJoachim W Engels
Mar 16, 2011·Journal of Separation Science·Virginia García-CañasAlejandro Cifuentes
Jun 14, 2006·Analytical Biochemistry·Andrey SemenyukMarek Kwiatkowski
Apr 23, 2008·Current Protocols in Nucleic Acid Chemistry·R T Pon
Jul 11, 2000·Nature Biotechnology·M NilssonU Landegren
Jan 1, 2015·Microarrays·Martin WittFrank Stahl
Oct 9, 2004·BioTechniques·Rowena F SternCaroline Mackie Ogilvie

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