Synthesis of gamma-carboxylated polypeptides by alpha-cells of the pancreatic islets

Biochemical and Biophysical Research Communications
L M StenbergM A Brown

Abstract

gamma-Carboxylated proteins were detected in normal human pancreas by immunohistochemistry with a monoclonal antibody (M3B) specific for gamma-carboxyglutamyl residues. Staining appeared to be localized to the glucagon-secreting alpha-cells in the islets of Langerhans. Consistent with this, sections from a glucagonoma were stained much more intensely with the M3B antibody than those from an insulinoma. A murine alpha-cell line (alphaTC1 Clone 9) was cultured and gamma-carboxylated polypeptides, identified immunologically as prothrombin, protein S and (tentatively) Gas6, were isolated from the intracellular compartment by chromatography on an M3B-coupled resin. As in liver, prothrombin is synthesized by alpha-cells as a gamma-carboxylated zymogen that can be cleaved by ecarin to form an active serine protease that is inhibited by hirudin. The pancreas thus appears to be a novel site of synthesis for certain vitamin K-dependent proteins.

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Citations

Apr 30, 2009·Annual Review of Nutrition·Sarah L Booth
Apr 21, 2012·Advances in Nutrition·Caren M GundbergSarah L Booth
Jun 9, 2004·Biochemical and Biophysical Research Communications·Diana D H ThomasGuy E Groblewski
Aug 14, 2008·Diabetes Care·Makiko YoshidaSarah L Booth
Sep 22, 2015·The Cochrane Database of Systematic Reviews·Louise HartleyKaren Rees
Sep 30, 2014·Biochemical and Biophysical Research Communications·Sachiko ShibaSatoshi Inoue
Dec 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·M A BrownL M Stenberg

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