PMID: 6411471Sep 15, 1983Paper

Synthesis of glycosaminoglycans by cloned bovine endothelial cells cultured on collagen gels

European Journal of Biochemistry
D J WinterbourneJ T Gallagher

Abstract

Sulphated glycosaminoglycans have been analysed in cloned bovine aortic endothelial cells cultured on collagen gels after incubation with [3H]glucosamine and Na2(35)SO4. Radioactive products were analysed in the culture medium, in sequential collagenase and trypsin extracts of the cell monolayer and the associated extracellular matrix, and in the remaining viable cells. Heparan sulphate and chondroitin sulphate were found in each compartment: the heparan sulphate had a low degree of sulphation (approximately 0.4 N-sulphate and 0.2 O-sulphate groups per disaccharide unit on average). In the nitrous acid scission products of heparan sulphate, O-sulphated substituents were confined to disaccharide and tetrasaccharide fragments, indicating that local regions of the chain (which might be susceptible to excission by the platelet endoglycosidase) are highly sulphated. Only minor structural differences in heparan sulphate were observed between the various compartments. In contrast the chondroitin sulphate found in the collagenase extract had a higher iduronic acid content than corresponding material in the trypsin extract and the culture medium, indicating that collagenase and trypsin may extract glycosaminoglycans from different regio...Continue Reading

References

Jan 1, 1978·Annual Review of Biochemistry·U Lindahl, M Höök
Jan 1, 1976·Annals of the New York Academy of Sciences·N SimionescuG E Palade
Jan 1, 1974·The Biochemical Journal·M HöökP H Iverius
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Citations

Jul 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A M Schor, S L Schor
Apr 1, 1990·International Journal of Biological Macromolecules·K R JergP Friedl
Mar 1, 1986·The Journal of Cell Biology·M G Kinsella, T N Wight
Jan 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·T N Wight
May 11, 1995·Biochimica Et Biophysica Acta·D R HiscockJ T Gallagher
May 12, 1995·Biochimica Et Biophysica Acta·D A Pye, S Kumar
May 30, 1985·Biochimica Et Biophysica Acta·T BârzuJ Caen

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