Synthesis of grafted phosphorylcholine polymer layers as specific recognition ligands for C-reactive protein focused on grafting density and thickness to achieve highly sensitive detection

Physical Chemistry Chemical Physics : PCCP
Yuri KamonToshifumi Takeuchi

Abstract

We studied the effects of layer thickness and grafting density of poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) thin layers as specific ligands for the highly sensitive binding of C-reactive protein (CRP). PMPC layer thickness was controlled by surface-initiated activators generated by electron transfer for atom transfer radical polymerization (AGET ATRP). PMPC grafting density was controlled by utilizing mixed self-assembled monolayers with different incorporation ratios of the bis[2-(2-bromoisobutyryloxy)undecyl] disulfide ATRP initiator, as modulated by altering the feed molar ratio with (11-mercaptoundecyl)tetra(ethylene glycol). X-ray photoelectron spectroscopy and ellipsometry measurements were used to characterize the modified surfaces. PMPC grafting densities were estimated from polymer thickness and the molecular weight obtained from sacrificial initiator during surface-initiated AGET ATRP. The effects of thickness and grafting density of the obtained PMPC layers on CRP binding performance were investigated using surface plasmon resonance employing a 10 mM Tris-HCl running buffer containing 140 mM NaCl and 2 mM CaCl2 (pH 7.4). Furthermore, the non-specific binding properties of the obtained layers were investig...Continue Reading

References

May 30, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·B ShineC B Wood
Dec 26, 2001·Chemical Reviews·K Matyjaszewski, J Xia
Mar 18, 2005·Journal of the American Chemical Society·Ke MinKrzysztof Matyjaszewski
Apr 14, 2005·Chemical Reviews·J Christopher LoveGeorge M Whitesides
Oct 12, 2005·Archives of Internal Medicine·Russell P Tracy, Lewis H Kuller
Mar 21, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Krzysztof MatyjaszewskiAndy Kusumo
Oct 1, 2008·Journal of Internal Medicine·J P CasasM B Pepys
Aug 14, 2010·Colloids and Surfaces. B, Biointerfaces·Yuuki Inoue, Kazuhiko Ishihara
Oct 7, 2011·Chemical Communications : Chem Comm·Eunjoo KimSang Won Jeong
Jan 1, 2013·Biosensors & Bioelectronics·Akiko MurataToshifumi Takeuchi
Jan 1, 2012·Progress in Polymer Science·Daniel J SiegwartKrzysztof Matyjaszewski
Jan 9, 2014·Clinical Chemistry and Laboratory Medicine : CCLM·Stefanie A DriegheJoris R Delanghe
Feb 15, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shun-Fa YangPo-Hui Wang
Apr 5, 2014·Advanced Healthcare Materials·Tatsuro GodaYuji Miyahara
Jul 26, 2014·Journal of Bioscience and Bioengineering·Shogo SasakiToshifumi Takeuchi

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Citations

Dec 15, 2015·Chemical Communications : Chem Comm·Ryo MatsuuraToshifumi Takeuchi
Apr 9, 2021·Colloids and Surfaces. B, Biointerfaces·Yuhao MaLarry D Unsworth
Aug 30, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Linbo HanHongbo Zeng
Jun 27, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Tetsuro SaekiToshifumi Takeuchi

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