Synthesis of highly selective fluorescent peptide probes for metal ions: tuning selective metal monitoring with secondary structure

Bioorganic & Medicinal Chemistry
Bishnu Prasad Joshi, Keun-Hyeung Lee

Abstract

Metal selective fluorescent peptide probes (dansyl-Cys-X-Gly-His-X-Gly-Glu-NH2, X = Pro or Gly) were developed by synthesizing peptides containing His, Cys, and Glu residues with Pro-Gly sequence to stabilize a turn structure and Gly-Gly sequence to adopt a random coil. The probe containing two Gly-Gly sequences exhibited marked selectivity only for Cu2+ over 13 metal ions including competitive transition and Group I and II metal ions under physiological buffer condition. In contrast, the probe containing double Pro-Gly sequences showed high selectivity for Zn2+. The peptide probe containing one Pro-Gly sequence exhibited selectivity for Zn2+ and Cu2+. CD spectra indicated that the secondary structure of the probes played an important role in the selective metal monitoring and a pre-organized secondary structure is not required for the selective detection of Cu2+ ion, but is required for the detection of Zn2+. We investigated and characterized the binding affinity, binding stoichiometry, reversibility, and pH sensitivity of the peptide probes.

References

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Citations

Jun 26, 2013·Journal of Inorganic Biochemistry·Attila JancsóRóbert Berkecz
Feb 14, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Tao YangLin Zhang
Sep 29, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Peng WangYu Tang
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May 22, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Levente I SzekeresAttila Jancsó
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Nov 21, 2020·Journal of Fluorescence·Shuaibing YuLianzhi Li

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