PMID: 6159584Sep 25, 1980Paper

Synthesis of human fibroblast interferon by E. coli

Nucleic Acids Research
D V GoeddelS Pestka

Abstract

A cDNA library was constructed using mRNA from human fibroblasts induced with poly(I):poly(C). A bacterial clone containing fibroblast interferon cDNA sequences was identified by hybridization to a cDNA probe synthesized using deoxyoligonucleotide primers which hybridize to fibroblast interferon mRNA specifically. Expression plasmids were constructed which permitted the synthesis in E. coli of 8 x 10(7) units of human fibroblast interferon per liter of culture. The bacterially produced fibroblast interferon is indistinguishable from authentic human fibroblast interferon by several criteria.

References

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Citations

Feb 15, 2001·Biopolymers·S Pestka
Jan 1, 1981·Immunogenetics·A A ReyesR B Wallace
Mar 1, 1988·Molecular & General Genetics : MGG·J YamayaT Ohno
Aug 6, 2008·Applied Biochemistry and Biotechnology·Dasari Venkata Krishna RaoAdibhatla Kali Satya Bhujanga Rao
Jan 1, 1982·Pharmacology & Therapeutics·A M AttallahJ C Petricciani
Jan 1, 1985·Pharmacology & Therapeutics·J A Langer, S Pestka
Oct 1, 1981·Journal of Virological Methods·M De LeyP De Somer
May 1, 1983·Gene·I PalvaC Weissmann
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S V SuggsK Itakura
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·N B Raj, P M Pitha
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D OwerbachR M Lawn
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S Ohno, T Taniguchi
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A R DavisD G Kleid
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R M LawnD V Goeddel
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P M PithaG S Hayward
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M CombR Crea
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J M TrentR M Lawn
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Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S CabillyH L Heyneker
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D F MarkL S Lin
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D G Yansura, D J Henner
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E JayM Dawood
Jan 1, 1984·Biotechnology & Genetic Engineering Reviews·M D Edge, R Camble
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Jan 1, 1982·Journal of Interferon Research·E Knight, D Fahey
Jan 1, 1982·Journal of Interferon Research·E Dworkin-RastlP Swetly

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