PMID: 6171427Nov 1, 1981Paper

Synthesis of human interferon beta 1 in Escherichia coli infected by a lambda phage recombinant containing a human genomic fragment

European Journal of Biochemistry
Y Y MoryM Revel

Abstract

DNA from a human adult was fragmented by partial digestion with restriction endonuclease EcoRI and cloned in lambda Charon 4A. Clone C15, with a human DNA insert of 17 X 10(3) bases, was identified as containing a gene for the fibroblast interferon, interferon beta 1. Restriction mapping shows that this gene, located on a 1840-base EcoRI fragment, is not interrupted by introns. Moreover, we show that this human genomic DNA fragment is able to direct the synthesis of active human interferon beta 1 in Escherichia coli. Interferon activity of up to 7 X 10(6) U/l was recovered from phage lysates by chromatography on Cibacron blue--Sepharose, and had the same immunological properties and species specificity as interferon produced by human fibroblasts.

References

Jan 1, 1979·Methods in Enzymology·F Bolivar, K Backman
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B Hohn, K Murray
Jun 14, 1979·Nature·O Mercereau-Puijalon, P Kourilsky
Jan 1, 1979·Methods in Enzymology·S A NarangR Brousseau
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K BackmanW Gilbert
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M Grunstein, D S Hogness
Jul 2, 1973·European Journal of Biochemistry·M Gross-BellardP Chambon
Jun 13, 1966·Biochemical and Biophysical Research Communications·D T Denhardt
Jun 1, 1980·Gene·T TaniguchiM Muramatsu
Sep 25, 1980·Nucleic Acids Research·D V GoeddelS Pestka
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T TaniguchiM Muramatsu
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T TaniguchiM Ptashne
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S SteinS Pestka
Jan 24, 1981·Nucleic Acids Research·M HoughtonN H Carey
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J WeissenbachM Revel
Mar 11, 1981·Nucleic Acids Research·R M LawnD V Goeddel
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert

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Citations

Jan 1, 1984·Human Genetics·L HenryC Junien
Jan 1, 1983·Annals of the New York Academy of Sciences·Y ChernajovskyM Revel
Aug 1, 1994·Journal of Interferon Research·G Allen, M O Diaz
Jan 1, 1984·Biotechnology & Genetic Engineering Reviews·M D Edge, R Camble
Mar 1, 1985·Molecular and Cellular Biology·S I FeinsteinM Revel

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