Synthesis of lens capsule and plasma membrane glycoproteins by lens epithelial cells and fibers in the rat

The American Journal of Anatomy
A Haddad, G C Bennett

Abstract

The lens of the eye possesses a capsule which is a greatly hypertrophied basement membrane. To investigate the synthesis of glycoproteins destined for this capsule, 3H-fucose was injected into the vitreous body of intact rats weighing approximately 200 gm. The animals were killed from 10 min to 14.5 months later, and their lenses were processed for electron microscope radioautography. At 10 min after injection, more than 58% of the silver grains were localized to the Golgi apparatus of the lens epithelial cells. By day 1, the heaviest sites of reaction were the plasma membrane (more than 50% of total label), the basal cytoplasm, and the adjacent lens capsule, where a heavy band of reaction was seen. The remainder of the capsule exhibited a lighter diffuse reaction. In the lens fibers, the label was at first localized to clusters of vesicles but then migrated to the plasma membrane and to the region of the capsule adjacent to the basal surface of these fibers. Light microscope radioautographs of the lens capsule at later time intervals revealed that by 1 month after injection the diffuse reaction had disappeared, and only the strongly labeled band remained. By 14.5 months after injection, this band had migrated partially across ...Continue Reading

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Citations

Nov 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·R de Iongh, J W McAvoy
Feb 1, 1991·Journal of Electron Microscopy Technique·G Bennett, G Wild
Oct 25, 2003·Progress in Retinal and Eye Research·Susanne Krag, Troels T Andreassen
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Aug 12, 1999·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·E C Castro, L Cesar Peres
Dec 1, 1989·Current Eye Research·C M Parmigiani, J W McAvoy
Jun 1, 1991·Current Eye Research·C M Parmigiani, J W McAvoy

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