Synthesis of Magnetic Nanoparticles Stabilized by Magnetite-Binding Protein for Targeted Delivery to Cancer Cells

Doklady. Biochemistry and Biophysics
P A KotelnikovaR V Petrov

Abstract

A new method for obtaining biomodified magnetite nanoparticles for targeted delivery to cells was developed. The method is based on the use of the C-terminal fragment of the Mms6 protein, which is involved in the magnetite biomineralization during the synthesis of magnetosomes in magnetotactic bacteria Magnetospirillum magneticum AMB-1, and the barnase*barstar high-affinity protein pair. The Mms6 protein fragment is required for stabilizing magnetite, and the barnase*barstar pair mediates the interaction between nanoparticles and the component for modification. The efficiency of this method was confirmed in the synthesis of magnetite nanoparticles recognizing the HER2/neu tumor marker and in the selective labeling of HER2/neu with these nanoparticles on the surface of cancer cells.

References

Dec 24, 2002·The Journal of Biological Chemistry·Atsushi ArakakiTadashi Matsunaga
Nov 25, 2003·Nature Biotechnology·Sergey M DeyevAndreas Plückthun
Aug 12, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sergey M Deyev, Ekaterina N Lebedenko
Aug 19, 2008·Journal of Molecular Biology·Daniel SteinerAndreas Plückthun
Aug 19, 2014·Nature Nanotechnology·Maxim P NikitinPetr I Nikitin
Oct 13, 2016·Scientific Reports·E V KhaydukovV Ya Panchenko

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Citations

May 8, 2021·Frontiers in Chemistry·Kumar Sachin, Santosh Kumar Karn
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Victoria Olegovna ShipunovaSergey Mikhailovich Deyev

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