Synthesis of methyl alpha-D-glucooligosaccharides by entrapped dextransucrase from Leuconostoc mesenteroides B-1299

Journal of Biotechnology
Aránzazu Gómez de SeguraFrancisco J Plou

Abstract

The synthesis of methyl alpha-D-glucooligosaccharides, using sucrose as glucosyl donor and methyl alpha-D-glucopyranoside as acceptor, was studied with dextransucrase from Leuconostoc mesenteroides NRRL B-1299. The enzyme was immobilized by entrapment in alginate. By NMR and mass spectrometry we identified three homologous series (S1-S3) of methyl alpha-D-glucooligosaccharides. Series S2 and S3 were characterized by the presence of alpha(1-->2) linkages, in combination with alpha(1-->6) bonds. Two parameters, sucrose to acceptor concentration ratio (S/A) and the total sugar concentration (TSC) determined the yield of methyl alpha-D-glucooligosaccharides. The maximum concentration achieved of the first acceptor product, methyl alpha-D-isomaltoside, was 65 mM using a S/A 1:4 and a TSC of 336 g l(-1). When increasing temperature, a shift of selectivity towards compounds containing alpha(1-->2) bonds was observed. The formation of leucrose as a side process was very significant (reaching values of 32 g l(-1)) at high sucrose concentrations.

Citations

May 22, 2009·Biotechnology Letters·Young-Min KimDoman Kim
Jan 23, 2016·Applied Microbiology and Biotechnology·Vladimír KrasňanMartin Rebroš
Aug 23, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Natália G GraebinRafael C Rodrigues
Jul 18, 2009·Biotechnology and Bioengineering·David SvenssonPatrick Adlercreutz
Mar 15, 2011·Journal of Agricultural and Food Chemistry·Ana I Ruiz-MatuteRobert A Rastall
Jul 10, 2018·Organic Letters·Feiqing DingYukishige Ito

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