PMID: 9433474Jan 20, 1998Paper

Synthesis of mono- and bifunctional peptide-dextran conjugates for the immobilization of peptide antigens on ELISA plates: properties and application

Journal of Immunological Methods
M BöcherU Bilitewski

Abstract

Dextran has been used as a carrier molecule for the synthesis of monofunctional peptide-dextran conjugates. The immunodetection of such carrier immobilized peptides on ELISA plates was compared to that of peptides adsorbed directly to immunoplates. The main features observed with peptide-dextran conjugates were as follows: only small amounts of peptide (1-2 mg) were necessary for coupling via alpha- or epsilon-amino groups to NaIO4-activated dextran (4 mg); the coupling yield was up to 68%; an amino acid analysis of the conjugate enabled the amount of carrier immobilized peptide to be calculated; an estimated 15-17 peptides were bound per dextran molecule (MW 73,500); using a carbohydrate as carrier reduces the possibility of non-specific interactions because no hydrophobic or ionic sites and no protein-like epitopes exist on the carrier apart from the peptide ligand. It can be assumed that some peptide ligands provide the forces for an interaction with the plate surface whereas other remain free for the interaction with the antibody. Thus, the detection with monoclonal anti-peptide antibodies allowed peptide-dextran conjugates to be used at coating concentrations of 1-3 nM peptide, corresponding to 0.6-2.6 ng peptide-dextran p...Continue Reading

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Citations

Dec 21, 2000·Journal of Immunological Methods·M J GómaraI Haro
Jun 1, 2005·Molecular and Cellular Biology·Antje BanningRegina Brigelius-Flohé
Oct 7, 2004·Biological & Pharmaceutical Bulletin·Li GanJian Ding
Jan 3, 2013·Journal of Immunological Methods·Benoît LiberelleGregory De Crescenzo
Oct 3, 2008·The Journal of Nutritional Biochemistry·Juliane HrdinaRegina Brigelius-Flohé
Mar 22, 2012·Expert Opinion on Drug Delivery·Jaleh Varshosaz
Mar 17, 2015·Journal of Immunoassay & Immunochemistry·Zhen-ming XieLi-sha Fang
Jun 12, 2012·Biochimica Et Biophysica Acta·Anna P KippRegina Brigelius-Flohé
Jun 27, 2015·Inflammatory Bowel Diseases·Franziska HillerAnna P Kipp
Mar 14, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Walter MeinlHansruedi Glatt
May 18, 2006·The Journal of Pharmacology and Experimental Therapeutics·Gerd BirkenmeierAngelika Schäfer
Oct 16, 2020·Redox Biology·Maria SchwarzAnna Patricia Kipp

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